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Level and Specificity of Antibodies Evoked by Crude and Purified Antigens of Poliovirus I and Echovirus 7

机译:脊髓灰质炎病毒I和回声病毒7的粗制和纯化抗原引起的抗体的水平和特异性

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Preparations of poliovirus I and echovirus 7, purified by density gradient centrifugation, liquid-phase partition, and anion exchange (diethylaminoethyl) chromatography, have been shown to evoke high antibody levels of substantial specificity in the complement-fixation assay. Certain practical aspects of the three purification methods are discussed. These results argue for the use of purified viral antigens, particularly in view of the simplicity of the purification methods now available.
机译:脊髓灰质炎病毒I和回声病毒7的制备物通过密度梯度离心,液相分配和阴离子交换(二乙基氨基乙基)色谱法纯化,在补体结合测定中已显示出高特异性的高抗体水平。讨论了三种纯化方法的某些实际方面。这些结果证明了使用纯化的病毒抗原,特别是考虑到目前可用的纯化方法的简便性。

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