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首页> 外文期刊>Applied Microbiology >Influence of Na+ on Synthesis of Macromolecules by a Marine Bacterium
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Influence of Na+ on Synthesis of Macromolecules by a Marine Bacterium

机译：Na +对海洋细菌合成大分子的影响

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Resting cells of Vibrio natriegens acquired the ability to take up 14C-labeled mannitol in media containing Na+ and K+. But, the cells took up a significant quantity of the label as well in the presence of 0.3 m K+ and no Na+. The label was distributed throughout the cells in both systems. Cells incubated in mannitol minimal culture medium proliferated and synthesized approximately nine times as much protein in the presence of Na+ and K+ as those incubated in the presence of mannitol and 0.3 m K+. The bacteria did not proliferate in the absence of Na+. Cells incubated in medium containing mannitol and Na+ and K+ synthesized approximately twice the quantity of deoxyribonucleic acid and ribonucleic acid as those incubated in medium containing mannitol and 0.3 m K+ but no Na+. A significant amount of mannitolbinding protein was synthesized in the membranes of V. natriegens incubated in the presence of mannitol and Na+ and K+, but only a small quantity was produced in medium containing mannitol and 0.3 m K+ but no Na+. A binding fraction comprising at least two proteins (both with molecular weight near 34,000) was isolated by gel electrophoresis from other components of a K2CO3-extract of membrane protein from mannitol-grown cells. This binding fraction mediated phosphorylation of mannitol at the expense of either adenosine triphosphate or phosphoenolpyruvate. It was then found that mannitol-grown, but not broth-grown, cells contained nicotinamide adenine dinucleotide-linked mannitol-1-phosphate dehydrogenase. Neither contained mannitol dehydrogenase.
机译：Natriegens弧菌的静止细胞获得了在含有Na +和K +的培养基中摄取14C标记的甘露醇的能力。但是，在存在0.3 m K +而没有Na +的情况下，细胞也吸收了大量的标记物。标签分布在两个系统的整个单元中。在Na +和K +存在下，在甘露醇基本培养基中孵育的细胞增殖和合成的蛋白质大约是在甘露醇和0.3 m K +存在下孵育的细胞的合成数量的九倍。在没有Na +的情况下，细菌不会增殖。在含甘露醇和Na +和K +的培养基中孵育的细胞合成的脱氧核糖核酸和核糖核酸的量约为在含甘露醇和0.3 m K +但不含Na +的培养基中孵育的细胞的两倍。在存在甘露醇和Na +和K +的条件下温育的纳氏弧菌的膜中合成了大量的甘露醇结合蛋白，但在含有甘露醇和0.3 m K +但不含Na +的培养基中仅产生少量甘露醇结合蛋白。通过凝胶电泳从甘露醇生长的细胞的膜蛋白的K 2 CO 3提取物的其他组分中通过凝胶电泳分离出包含至少两种蛋白（分子量均接近34,000）的结合级分。该结合部分以三磷酸腺苷或磷酸烯醇丙酮酸为代价介导甘露醇的磷酸化。然后发现甘露醇生长的，而不是肉汤生长的细胞含有烟酰胺腺嘌呤二核苷酸连接的甘露醇-1-磷酸脱氢酶。两者均不含甘露醇脱氢酶。

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