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Salmonellae as an Index of Pollution of Surface Waters

机译:沙门氏菌作为地表水污染指数

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Screening enrichments of surface water specimens by means of a polyvalent fluorescent antibody reagent for the salmonellae yielded approximately 60% more positive specimens than was obtained by cultural procedures. It is not known what fraction of the excess of fluorescent antibody-positive over culturally positive specimens represents staining of non-salmonellae or non-arizonae as opposed to the staining of non-cultivatable organisms of these two genera. Cotton gauze and rayon-polypropylene fiber swabs were equally sensitive for collecting salmonellae from the streams examined. Tetrathionate enrichment incubated at 41.5 C appeared to be superior to selenite-cystine for isolation of salmonellae from surface waters. Twenty-eight serotypes of Salmonella and two serotypes of Arizona were identified in the 121 positive specimens. In water rated moderately polluted, 65% of all specimens tested were positive; in minimally polluted waters, 38% were positive; and in unpolluted streams, 44% were positive.
机译:用多价荧光抗体试剂筛选沙门氏菌的地表水样品富集,比培养方法得到的阳性样品多约60%。与这两个属的不可耕种生物体的染色相反,尚不知道相对于文化阳性标本而言,过量的荧光抗体阳性部分代表非沙门氏菌或非伞形菌的染色。棉纱布和人造丝-聚丙烯纤维棉签对从所检查的溪流中收集沙门氏菌同样敏感。在从地表水中分离沙门氏菌时,在41.5 C下孵育的四硫代硫酸盐富集似乎优于亚硒酸盐-胱氨酸。在121份阳性标本中鉴定出28种沙门氏菌血清型和2种亚利桑那州血清型。在等级为中度污染的水中,所有测试样本中有65%为阳性。在最低污染的水中,阳性率为38%。在未污染的河流中,阳性率为44%。

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