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首页> 外文期刊>Applied Microbiology >Culture of Ethiopian Strains of Borrelia recurrentis
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Culture of Ethiopian Strains of Borrelia recurrentis

机译：轮枝疏螺旋体埃塞俄比亚菌株的培养

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A number of standard bacteriological media with supplements were tested for their ability to support in vitro growth of Ethiopian strains of Borrelia recurrentis. Propagation of 18 out of 21 strains occurred in Trypticase soy yeast broth to which bovine albumin (fraction V), N-acetyl glucosamine, and sodium pyruvate had been added. This medium supported a population of 107 organisms per ml and yielded a harvest of four to five times the original inoculum during the logarithmic phase of growth. Maximal yield varied from 1.4 × 107 to 3.4 × 107 organisms per ml. Generation time in optimal media was 11.3 h. Lesser multiplication of organisms occurred in other media tested. Strains from primary cultures were infective for the green monkey. Recovery of viable organisms from subculture has not been successful.
机译：测试了许多具有补充剂的标准细菌学培养基支持轮枝疏螺旋体埃塞俄比亚菌株体外生长的能力。在添加了牛白蛋白（馏分V），N-乙酰氨基葡萄糖和丙酮酸钠的胰蛋白水解酶大豆发酵液中，发生了21种菌株中的18种的繁殖。该培养基每毫升可容纳107种生物，在对数生长期，收获量是原始接种量的4至5倍。每毫升最大产量从1.4×107到3.4×107个生物。在最佳培养基中的产生时间为11.3小时。在其他测试的介质中发生的生物繁殖较少。来自原代培养的菌株对绿猴具有感染力。从亚培养中回收活生物体尚未成功。

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