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首页> 外文期刊>Applied Microbiology >Isolation and Characterization of a Thermotolerant Methanol-Utilizing Yeast
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Isolation and Characterization of a Thermotolerant Methanol-Utilizing Yeast

机译：耐热甲醇酵母的分离与表征

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摘要

A yeast capable of growth on methanol as its sole carbon-energy source was isoalted from soil samples and identified as a strain of Hansenula polymorpha. A continuous enrichment culture at 37 C with a simple mineral salts medium was used to select this organism. The isolate, designated DL-1, has a maximal specific growth rate of 0.22 per h, at pH 4.5 to 5.5 and temperatures of 37 to 42 C, in simple mineral salts medium with methanol (0.5%), biotin, and thiamine. Growth occurred in a chemostat at temperatures up to 50 C, with strong growth at 45 C. The maximal growth yield of the yeast on methanol was 0.36 g of dry cell weight per g of methanol, and the yield on oxygen was 0.37 g of dry cell weight per g of O2. Protein content of the isolate is 46%, and total nucleic acid content varies from 5.0 to 7.0% with increasing growth rate from 0.08 to 0.20 per h. The amino acid profile of this yeast protein indicates that it could serve as a good source of food protein. Feeding studies with rats show the yeast to have no toxic effects.
机译：从土壤样品中分离出能够在甲醇上作为其唯一碳能源来源生长的酵母，并将其鉴定为多形汉逊酵母菌株。使用简单的矿物盐培养基在37°C下连续富集培养，以选择该生物。在含有甲醇（0.5％），生物素和硫胺素的简单无机盐培养基中，命名为DL-1的分离物在pH 4.5至5.5和温度37至42 C的条件下，最大比生长速率为0.22 / h。在温度高达50℃的化学恒温器中发生生长，并在45℃强烈生长。酵母在甲醇上的最大生长产量为每克甲醇0.36 g干细胞重量，在氧气上的产量为0.37 g干细胞每克O2的细胞重量。分离物的蛋白质含量为46％，总核酸含量从5.0到7.0％不等，生长速率从每小时0.08到0.20递增。该酵母蛋白的氨基酸谱表明它可以作为食物蛋白的良好来源。对大鼠的喂养研究表明，该酵母没有毒性作用。

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