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Production of Staphylococcal Enterotoxins A, B, and C in Colloidal Dispersions

机译:胶体分散液中葡萄球菌肠毒素A,B和C的产生

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Larger amounts of enterotoxin were produced when Staphylococcus aureus S-6 was grown under still (nonshaken) conditions in a medium that was a paste or gel than were produced in a liquid dispersion with the same colloidal ingredient or in control basal broth (4% NZ Amine-NAK containing 50 μg of thiamine per 100 ml and 1 mg of niacin per 100 ml). Four colloidal ingredients were used which had been previously demonstrated to not support enterotoxin production in buffer. The effect of the type of dispersion occurred earlier than that of the colloidal ingredient, but interactions were found. This effect was not observed when the cells were grown with aeration (shaken). Four other strains of S. aureus followed a similar pattern for enterotoxins A, B, and C, although liquid and paste with cornstarch and carrageenan were the only media compared to the control broth. Enterotoxins A and B were produced earlier by S. aureus S-6, and much greater quantities of enterotoxins were produced for all strains when incubated shaken.
机译:当金黄色葡萄球菌S-6在静止(不晃动)条件下在糊状或凝胶状培养基中生长时,产生的肠毒素量要比在具有相同胶体成分的液体分散液中或在对照基础肉汤中产生的肠毒素量大(4%) NZ Amine-NAK,每100毫升含50微克硫胺素,每100毫升含1毫克烟酸。使用了四种胶体成分,先前已证明它们不支持缓冲液中肠毒素的产生。分散类型的影响比胶体成分的影响更早发生,但发现了相互作用。当细胞通气生长(摇动)时,未观察到这种效果。尽管与对照肉汤相比,液体和糊状玉米淀粉和角叉菜胶是唯一的培养基,但其他四种金黄色葡萄球菌的肠毒素A,B和C的模式也相似。肠毒素A和B是由金黄色葡萄球菌S-6较早产生的,摇动孵育后,所有菌株产生的肠毒素量要大得多。

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