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首页> 外文期刊>Applied Microbiology >Detection of Escherichia coli Antigens by a Latex Agglutination Test
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Detection of Escherichia coli Antigens by a Latex Agglutination Test

机译：乳胶凝集试验检测大肠杆菌抗原

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摘要

A latex particle agglutination technique to detect ethylenediaminetetraacetate-solubilized extracts from Escherichia coli and whole E. coli cells is described. The sensitivity of the serological test was found to be 0.5 to 2.5 ng for the solubilized antigens and 1.5 × 106 to 5.7 × 106 cells per ml for the particulate antigens. The test was 100 to 1,000 times more sensitive than the standard bacterial agglutination test. Furthermore, it detected E. coli antigens during all phases of bacterial growth, whereas the bacterial test detected the antigens only after the mid-log phase. No significant cross-reactivity was observed between latex-anti-E. coli preparations and heterologous bacterial strains used in the experimental procedure. A buffer formula containing fatty acid-free bovine albumin prevented nonspecific aggregation of the latex particles.
机译：描述了一种乳胶颗粒凝集技术，用于检测乙二胺四乙酸酯增溶的大肠杆菌和整个大肠杆菌细胞的提取物。血清学测试的灵敏度对溶解的抗原为0.5至2.5 ng / ml，对颗粒状抗原为1.5×106至5.7×106细胞。该测试的灵敏度是标准细菌凝集测试的100到1,000倍。此外，它在细菌生长的所有阶段都检测到大肠杆菌抗原，而细菌测试仅在对数中期后才检测到抗原。乳胶-抗-E之间未观察到明显的交叉反应。实验过程中使用的大肠杆菌制剂和异源细菌菌株。含有无脂肪酸的牛白蛋白的缓冲液配方可防止胶乳颗粒的非特异性聚集。

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