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首页> 外文期刊>Applied Microbiology >Importance of SigB for Listeria monocytogenes Static and Continuous-Flow Biofilm Formation and Disinfectant Resistance
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Importance of SigB for Listeria monocytogenes Static and Continuous-Flow Biofilm Formation and Disinfectant Resistance

机译：SigB对单核细胞增生性李斯特菌的重要性静态和连续流生物膜形成和抗药性

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Listeria monocytogenes is a food-borne pathogen that is able to form biofilms in food processing facilities. Biofilms are generally more resistant to antimicrobial agents, making it difficult to eradicate them during cleanup procedures. So far, little is known about the function of stress resistance mechanisms in biofilm formation and their resistance to disinfectants. In this study, we investigated the role of sigB , which encodes a major transcriptional regulator of stress response genes, in L. monocytogenes static and continuous-flow biofilm formation and its function in the resistance of biofilm cells to the disinfectants benzalkonium chloride and peracetic acid. Quantitative real-time PCR and promoter reporter studies showed that sigB is activated in static and continuous-flow biofilms. Biofilm formation studies using an in-frame sigB deletion mutant and complementation mutant showed that the presence of SigB is required to obtain wild-type levels of both static and continuous-flow biofilms. Finally, disinfection treatments of planktonically grown cells and cells dispersed from static and continuous-flow biofilms showed that SigB is involved in the resistance of both planktonic cells and biofilms to the disinfectants benzalkonium chloride and peracetic acid.
机译：单核细胞增生李斯特菌是一种食物传播的病原体，能够在食品加工设施中形成生物膜。生物膜通常对抗菌剂具有更强的抵抗力，因此很难在清洁过程中将其根除。迄今为止，关于抗应力机制在生物膜形成中的功能及其对消毒剂的抗性知之甚少。在这项研究中，我们调查了sigB在单核细胞增生李斯特氏菌静态和连续流动生物膜形成中的作用，它编码应激反应基因的主要转录调节因子，并且在生物膜细胞对消毒剂苯扎氯铵和过氧乙酸的抗性中起作用。实时定量PCR和启动子报告基因研究表明sigB在静态和连续流动的生物膜中被激活。使用框内sigB缺失突变体和互补突变体的生物膜形成研究表明，要获得野生型水平的静态和连续流动生物膜都需要SigB的存在。最后，对浮游生物生长的细胞以及从静态和连续流动生物膜分散的细胞进行的消毒处理表明，SigB参与了浮游细胞和生物膜对消毒剂苯扎氯铵和过氧乙酸的抗性。

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