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首页> 外文期刊>Applied Microbiology >Activation of the Ustilagic Acid Biosynthesis Gene Cluster in Ustilago maydis by the C2H2 Zinc Finger Transcription Factor Rua1
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Activation of the Ustilagic Acid Biosynthesis Gene Cluster in Ustilago maydis by the C2H2 Zinc Finger Transcription Factor Rua1

机译：C2H2锌指转录因子Rua1激活玉米til中鱼尿酸生物合成基因簇的激活

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摘要

The phytopathogenic basidiomycetous fungus Ustilago maydis secretes, under conditions of nitrogen starvation, large amounts of the biosurfactant ustilagic acid (UA). This secreted cellobiose glycolipid is toxic for many microorganisms and confers biocontrol activity to U. maydis . Recently, a large gene cluster that is responsible for UA biosynthesis was identified. Here, we show that expression of all cluster genes depends on Rua1, a nuclear protein of the C_(2)H_(2) zinc finger family, whose gene is located within the gene cluster. While deletion of rua1 results in complete loss of UA production, overexpression of rua1 promotes increased UA synthesis even in the presence of a good nitrogen source. Bioinformatic analysis allowed us to identify a conserved sequence element that is present in the promoters of all structural genes involved in UA biosynthesis. Deletion analysis of several promoters within the cluster revealed that this DNA element serves as an upstream activating sequence (UAS) and mediates Rua1-dependent expression. We used the yeast one-hybrid system to demonstrate specific recognition of this DNA element by Rua1. Introduction of nucleotide exchanges into the consensus sequence interfered with Rua1-dependent activation, suggesting that this sequence element acts as a direct binding site for Rua1.
机译：在氮饥饿的条件下，植物致病性担子菌真菌Ustilago可能会分泌大量生物表面活性剂奥司他酸（UA）。这种分泌的纤维二糖糖脂对许多微生物有毒害，赋予了U. maydis生物控制活性。最近，鉴定了负责UA生物合成的大基因簇。在这里，我们显示所有簇基因的表达取决于Rua1，Rua1是C_（2）H_（2）锌指家族的核蛋白，其基因位于基因簇内。尽管rua1的缺失会导致UA生产的完全丧失，但rua1的过表达即使在存在良好氮源的情况下也会促进UA合成的增加。生物信息学分析使我们能够鉴定出保守序列元素，该序列元素存在于UA生物合成的所有结构基因的启动子中。群集中几个启动子的缺失分析表明，该DNA元件充当上游激活序列（UAS）并介导Rua1依赖性表达。我们使用酵母单杂交系统来证明Rua1对这种DNA元件的特异性识别。将核苷酸交换引入共有序列会干扰Rua1依赖的激活，这表明该序列元件充当Rua1的直接结合位点。

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