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首页> 外文期刊>Applied Microbiology >Randomly Amplified Polymorphic DNA Reveals Tight Links between Viruses and Microbes in the Bathypelagic Zone of the Northwestern Mediterranean Sea
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Randomly Amplified Polymorphic DNA Reveals Tight Links between Viruses and Microbes in the Bathypelagic Zone of the Northwestern Mediterranean Sea

机译：随机扩增的多态性DNA揭示了西北地中海深水带中病毒与微生物之间的紧密联系

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The study site located in the Mediterranean Sea was visited eight times in 2005 and 2006 to collect samples from the epipelagic (5 m), mesopelagic (200 m, 600 m), and bathypelagic (1,000 m, 2,000 m) zones. Randomly amplified polymorphic DNA PCR (RAPD-PCR) analysis was used to obtain fingerprints from microbial and viral size fractions using two different primers each. Depending on the primer used, the number of bands in the water column varied between 12 to 24 and 6 to 19 for the microbial size fraction and between 16 to 26 and 8 to 22 for the viral size fraction. The majority of sequences from the microbial fraction was related to Alphaproteobacteria , Cyanobacteria , Gammaproteobacteria , Firmicutes , and Eukaryota . Only 9% of sequences obtained from the viral fraction were of identifiable viral origin; however, 76% of sequences had no close relatives in the nr database of GenBank. Only 20.1% of complete phage genomes tested in silico resulted in potential RAPD-PCR products, and only 12% of these were targeted by both primers. Also, in silico analysis indicated that RAPD-PCR profiles obtained by the two different primers are largely representative of two different subsets of the viral community. Also, correlation analyses and Mantel tests indicate that the links between changes in the microbial and viral community were strongest in the bathypelagic. Thus, these results suggest a strong codevelopment of virus and host communities in deep waters. The data also indicate that virus communities in the bathypelagic zone can exhibit substantial temporal dynamics.
机译：2005年和2006年，对位于地中海的研究地点进行了八次访问，以收集上表层（5 m），中古生界（200 m，600 m）和水上表层（1,000 m，2,000 m）区域的样本。随机扩增多态性DNA PCR（RAPD-PCR）分析用于使用两种不同的引物从微生物和病毒大小级分中获取指纹。根据所用引物的不同，水柱中条带的数量对于微生物尺寸级分在12至24至6至19之间，对于病毒尺寸级分在16至26至8至22之间变化。来自微生物部分的大多数序列与Alphaproteobacteria，Cyanobacteria，Gammaproteobacteria，Firmicutes和Eukaryota有关。从病毒级分获得的序列中只有9％具有可识别的病毒来源;然而，在GenBank的nr数据库中，有76％的序列没有近亲。在计算机上测试的完整噬菌体基因组中，只有20.1％产生了潜在的RAPD-PCR产物，两种引物仅靶向其中的12％。另外，计算机分析表明，由两种不同的引物获得的RAPD-PCR图谱在很大程度上代表了病毒群落的两个不同的子集。而且，相关分析和Mantel测试表明，在深浮层中，微生物和病毒群落变化之间的联系最强。因此，这些结果表明在深水区，病毒和宿主群落之间有很强的共同发展。数据还表明，深海带中的病毒群落可表现出明显的时间动态。

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