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首页> 外文期刊>Applied Microbiology >Construction of Self-Transmissible Green Fluorescent Protein-Based Biosensor Plasmids and Their Use for Identification of N-Acyl Homoserine-Producing Bacteria in Lake Sediments
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Construction of Self-Transmissible Green Fluorescent Protein-Based Biosensor Plasmids and Their Use for Identification of N-Acyl Homoserine-Producing Bacteria in Lake Sediments

机译：基于自发性绿色荧光蛋白的生物传感器质粒的构建及其在湖沉积物中N-酰基同型丝氨酸生产菌的鉴定中

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摘要

Many bacteria utilize quorum sensing (QS) systems to communicate with each other by means of the production, release, and response to signal molecules. N -Acyl homoserine lactone (AHL)-based QS systems are particularly widespread among the Proteobacteria , in which they regulate various functions. It has become evident that AHLs can also serve as signals for interspecies communication. However, knowledge on the impact of AHLs for the ecology of bacteria in their natural habitat is scarce, due mainly to the lack of tools that allow the study of QS in bacterial communities in situ . Here, we describe the construction of self-mobilizable green fluorescent protein (GFP)-based AHL sensors that utilize the conjugation and replication properties of the broad-host-range plasmid RP4. We show that these novel AHL sensor plasmids can be easily transferred to different bacterial species by biparental mating and that they give rise to green fluorescent cells in case the recipient is an AHL producer. We also demonstrate that these sensor plasmids are capable of self-spreading within mixed biofilms and are a suitable tool for the identification of AHL-producing bacteria in lake sediment.
机译：许多细菌利用群体感应（QS）系统通过信号分子的产生，释放和响应相互通信。基于N-酰基高丝氨酸内酯（AHL）的QS系统在变形杆菌中尤为普遍，它们调节着各种功能。显然，AHL还可以用作种间通信的信号。但是，关于AHL对自然栖息地细菌生态学影响的知识很少，这主要是由于缺乏能够在细菌群落中进行QS研究的工具。在这里，我们描述了利用自宿主绿色荧光蛋白（GFP）的AHL传感器的构建，该传感器利用了宽宿主范围质粒RP4的结合和复制特性。我们表明这些新的AHL传感器质粒可以很容易地通过双亲交配转移到不同的细菌种类，并且如果接收者是AHL生产者，它们会产生绿色荧光细胞。我们还证明了这些传感器质粒能够在混合生物膜内自我传播，并且是鉴定湖沉积物中产生AHL细菌的合适工具。

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