Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose

Allison Riederer; Taichi E. Takasuka; Shin-ichi Makino; David M. Stevenson; Yury V. Bukhman; Nathaniel L. Elsen; Brian G. Fox

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Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose

机译：通过在纤维素或纤维二糖上的恒化器培养物的微阵列分析确定的在热纤梭菌中的全局基因表达模式

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A microarray study of chemostat growth on insoluble cellulose or soluble cellobiose has provided substantial new information on Clostridium thermocellum gene expression. This is the first comprehensive examination of gene expression in C. thermocellum under defined growth conditions. Expression was detected from 2,846 of 3,189 genes, and regression analysis revealed 348 genes whose changes in expression patterns were growth rate and/or substrate dependent. Successfully modeled genes included those for scaffoldin and cellulosomal enzymes, intracellular metabolic enzymes, transcriptional regulators, sigma factors, signal transducers, transporters, and hypothetical proteins. Unique genes encoding glycolytic pathway and ethanol fermentation enzymes expressed at high levels simultaneously with previously established maximal ethanol production were also identified. Ranking of normalized expression intensities revealed significant changes in transcriptional levels of these genes. The pattern of expression of transcriptional regulators, sigma factors, and signal transducers indicates that response to growth rate is the dominant global mechanism used for control of gene expression in C. thermocellum. .

机译：一项关于不溶性纤维素或可溶性纤维二糖上的恒化生长的微阵列研究为热纤梭菌基因表达提供了重要的新信息。这是在确定的生长条件下对热纤梭菌基因表达的首次全面检查。从3,189个基因中的2,846个检测到表达，回归分析显示348个基因的表达模式变化与生长速率和/或底物相关。成功建模的基因包括支架蛋白和纤维素酶，细胞内代谢酶，转录调节因子，σ因子，信号转导子，转运蛋白和假定蛋白的基因。还鉴定了编码糖酵解途径和乙醇发酵酶的高表达的独特基因，同时与先前确定的最大乙醇产量同时表达。归一化表达强度的排名揭示了这些基因的转录水平的显着变化。转录调节因子，sigma因子和信号转导子的表达模式表明，对生长速率的响应是控制热纤梭菌基因表达的主要全局机制。。

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《Applied Microbiology》 |2011年第4期|共11页
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Allison Riederer; Taichi E. Takasuka; Shin-ichi Makino; David M. Stevenson; Yury V. Bukhman; Nathaniel L. Elsen; Brian G. Fox;
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1. Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose [J] . Allison Riederer, Taichi E. Takasuka, Shin-ichi Makino, Applied and Environmental Microbiology . 2011,第4期

机译：通过在纤维素或纤维二糖上的恒化器培养物的微阵列分析确定的在热纤梭菌中的全局基因表达模式
2. Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose [J] . Allison Riederer, Taichi E. Takasuka, Shin-ichi Makino, Applied Microbiology . 2011,第4期

机译：通过在纤维素或纤维二糖上的恒化器培养物的微阵列分析确定的在热纤梭菌中的全局基因表达模式
3. Expression of 17 Genes in Clostridium thermocellum ATCC 27405 during Fermentation of Cellulose or Cellobiose in Continuous Culture [J] . David M. Stevenson, Paul J. Weimer Applied and Environmental Microbiology . 2005,第8期

机译：连续培养纤维素或纤维二糖发酵过程中热纤梭菌ATCC 27405中17个基因的表达
4. Quantitative assessment of H2 and CO2 supersaturation during thermophilic cellobiose fermentation with Clostridium thermocellum [C] . Warren Blunt, Daniel Gapes, Richard Sparling, Annual International Meeting of the American Society of Agricultural and Biological Enginners . 2015

机译：用梭菌热团聚嗜热纤维发酵期间H2和CO2过饱和的定量评估
5. Expression changes of canine transporter targeting-related genes during epithelial cells differentiation determined by microarray analysis and RT-PCR. [D] . Xu, Xiaofan. 2014

机译：通过微阵列分析和RT-PCR确定上皮细胞分化过程中犬转运蛋白靶向相关基因的表达变化。
6. Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose [O] . Allison Riederer, Taichi E. Takasuka, Shin-ichi Makino, 2011

机译：通过在纤维素或纤维二糖上的恒化器培养物的微阵列分析确定在热纤梭菌中的全球基因表达模式。
7. Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose▿ † [O] . Riederer, Allison, Takasuka, Taichi E., Makino, Shin-ichi, 2010

机译：通过对纤维素或纤维二糖上的恒化器培养物进行微阵列分析确定的热纤梭菌中的全局基因表达模式
8. Fermentation of Cellulose and Cellobiose by 'Clostridium thermocellum' in the Absence and Presence of 'Methanobacterium thermoautotrophicum'. (Thermophilic Cellulose Fermentation) [R] . Weimer, P. J. , Zeikus, J. G. 1975

机译：'热纤维菌（methanobacterium thermoautotrophicum）'缺乏和存在下'热纤梭菌'发酵纤维素和纤维二糖。（嗜热纤维素发酵）

Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose

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