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首页> 外文期刊>Applied Microbiology >Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species
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Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species

机译：含霉菌酸的细菌诱导链霉菌属物种的天然产物生物合成。

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Natural products produced by microorganisms are important starting compounds for drug discovery. Secondary metabolites, including antibiotics, have been isolated from different Streptomyces species. The production of these metabolites depends on the culture conditions. Therefore, the development of a new culture method can facilitate the discovery of new natural products. Here, we show that mycolic acid-containing bacteria can influence the biosynthesis of cryptic natural products in Streptomyces species. The production of red pigment by Streptomyces lividans TK23 was induced by coculture with Tsukamurella pulmonis TP-B0596, which is a mycolic acid-containing bacterium. Only living cells induced this pigment production, which was not mediated by any substances. T . pulmonis could induce natural-product synthesis in other Streptomyces strains too: it altered natural-product biosynthesis in 88.4% of the Streptomyces strains isolated from soil. The other mycolic acid-containing bacteria, Rhodococcus erythropolis and Corynebacterium glutamicum , altered biosynthesis in 87.5 and 90.2% of the Streptomyces strains, respectively. The coculture broth of T . pulmonis and Streptomyces endus S-522 contained a novel antibiotic, which we named alchivemycin A. We concluded that the mycolic acid localized in the outer cell layer of the inducer bacterium influences secondary metabolism in Streptomyces , and this activity is a result of the direct interaction between the mycolic acid-containing bacteria and Streptomyces . We used these results to develop a new coculture method, called the combined-culture method, which facilitates the screening of natural products.
机译：微生物产生的天然产物是药物发现的重要起始化合物。已从不同链霉菌种中分离出包括抗生素在内的次生代谢产物。这些代谢产物的产生取决于培养条件。因此，开发新的养殖方法可以促进新天然产品的发现。在这里，我们表明，含有霉菌酸的细菌可以影响链霉菌属物种中隐性天然产物的生物合成。与含霉菌酸的细菌肺部冢卡氏菌TP-B0596共培养可诱导青紫链霉菌TK23产生红色素。只有活细胞诱导这种色素生成，而色素没有任何物质介导。 。肺炎也可以在其他链霉菌菌株中诱导天然产物的合成：它改变了从土壤中分离出的88.4％链霉菌菌株的天然产物生物合成。其他含有霉菌酸的细菌红球菌和谷氨酸棒杆菌分别改变了87.5％和90.2％的链霉菌菌株的生物合成。 T的共培养液。肺炎和链霉菌内毒素S-522含有一种新型抗生素，我们将其命名为alchivemycinA。我们得出的结论是，位于诱导细菌外细胞层中的霉菌酸会影响链霉菌的次级代谢，而这种活性是直接相互作用的结果在含有霉菌酸的细菌和链霉菌之间。我们利用这些结果开发了一种新的共培养方法，称为联合培养方法，该方法可促进天然产物的筛选。

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