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首页> 外文期刊>Applied Microbiology >Requirement of a Tsp2-Type Tetraspanin for Laccase Repression and Stress Resistance in the Basidiomycete Cryptococcus neoformans
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Requirement of a Tsp2-Type Tetraspanin for Laccase Repression and Stress Resistance in the Basidiomycete Cryptococcus neoformans

机译：Tsp2-型四跨膜蛋白对新生乳杆菌隐球菌的漆酶抑制和抗逆性的要求

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摘要

Fungal laccases have been widely used in industry. The expression of laccase often is repressible by the primary carbon source glucose in many fungi. The underlying basis is largely unclear. We demonstrate here that a gene, TSP2 - 1 , was required for laccase repression by glucose in the basidiomycete Cryptococcus neoformans . TSP2 - 1 encodes a Tsp2-type tetraspanin. The disruption of TSP2 - 1 resulted in constant melanin formation and the expression of the laccase gene LAC1 . This derepression phenotype was restorable by 10 mM exogenous cyclic AMP (cAMP). A capsule defect in the mutant tsp2 - 1 Δ also was restored by cAMP. The results indicate an interaction of Tsp2-1 with the cAMP-dependent protein kinase A (PKA) pathway that has been shown to modulate laccase repression and capsule biosynthesis in this fungus. Other roles of TSP2 - 1 , e.g., in maintaining cell membrane integrity and stress resistance, also were defined. This work reveals a Tsp2-1-dependent glucose repression in C. neoformans . The function of Tsp2-type tetraspanin Tsp2-1 is described for the first time.
机译：真菌漆酶已广泛用于工业中。在许多真菌中，漆酶的表达通常可被主要的碳源葡萄糖抑制。根本的基础还不清楚。我们在这里证明，一个基因，TSP2-1，需要由乳杆菌抑制新孢子隐球菌中的葡萄糖来抑制漆酶。 TSP2-1编码Tsp2型四跨膜蛋白。 TSP2-1的破坏导致恒定的黑色素形成和漆酶基因LAC1的表达。通过10 mM外源性环状AMP（cAMP）可以恢复这种抑制表型。 cAMP也恢复了突变体tsp2-1-Δ中的胶囊缺陷。结果表明，Tsp2-1与cAMP依赖性蛋白激酶A（PKA）途径的相互作用已显示出可调节该真菌中的漆酶阻抑和胶囊生物合成。还定义了TSP2-1的其他作用，例如在维持细胞膜完整性和抗逆性方面。这项工作揭示了新孢梭菌中Tsp2-1依赖性葡萄糖抑制。 Tsp2型四跨膜蛋白Tsp2-1的功能是第一次描述。

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