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首页> 外文期刊>Applied Microbiology >Differences in the Fecal Concentrations and Genetic Diversities of Campylobacter jejuni Populations among Individual Cows in Two Dairy Herds
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Differences in the Fecal Concentrations and Genetic Diversities of Campylobacter jejuni Populations among Individual Cows in Two Dairy Herds

机译：两个奶牛群中个体母牛空肠弯曲菌种群粪便浓度和遗传多样性的差异

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Dairy cows have been identified as common carriers of Campylobacter jejuni , which causes many of the human gastroenteritis cases reported worldwide. To design on-farm management practices that control the human infection sourced from dairy cows, the first step is to acquire an understanding of the excretion patterns of the cow reservoir. We monitored the same 35 cows from two dairy farms for C. jejuni excretion fortnightly for up to 12 months. The objective was to examine the concentration of C. jejuni and assess the genetic relationship of the C. jejuni populations excreted by individual cows. Significant differences ( P < 0.01) in C. jejuni fecal concentration were observed among the 35 cows, with median concentrations that varied by up to 3.6 log_(10) · g~(?1) feces. A total of 36 different genotypes were identified from the 514 positive samples by using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Although 22 of these genotypes were excreted by more than one cow, the analysis of frequencies and distribution of the genotypes by model-based statistics revealed a high degree of individuality in the C. jejuni population in each cow. The observed variation in the frequency of excretion of a genotype among cows and the analysis by multilocus sequence typing (MLST) of these genotypes suggest that excretion of C. jejuni in high numbers is due to a successful adaptation of a particular genotype to a particular cow's gut environment, but that animal-related factors render some individual cows resistant to colonization by particular genotypes. The reasons for differences in C. jejuni colonization of animals warrant further investigation.
机译：奶牛已被确认为空肠弯曲菌的常见携带者，空肠弯曲菌可导致全世界报道的许多人胃肠炎病例。要设计农场管理实践以控制源自奶牛的人类感染，第一步是要了解母牛水库的排泄方式。我们每两个星期对来自两个奶牛场的同样35头母牛进行空肠弯曲杆菌排泄监测，长达12个月。目的是检查空肠弯曲杆菌的浓度并评估个体母牛排泄的空肠弯曲杆菌种群的遗传关系。在这35头母牛中，空肠弯曲杆菌粪便浓度存在显着差异（P <0.01），中位浓度差异最大为3.6 log_（10）·g〜（？1）粪便。使用肠细菌重复基因间共有（ERIC）-PCR技术，从514个阳性样品中鉴定出总共36种不同的基因型。尽管其中22种基因型被多头母牛排泄，但通过基于模型的统计数据对基因型的频率和分布进行分析后发现，每头母牛的空肠弯曲菌种群具有高度的个体性。在奶牛中观察到的基因型排泄频率变化以及这些基因型的多基因座序列分型（MLST）分析表明，空肠弯曲杆菌的排泄数量高是由于特定基因型成功适应了特定的母牛肠道环境，但是与动物有关的因素使某些个体母牛对特定基因型的定殖具有抗性。空肠弯曲杆菌动物定植差异的原因值得进一步研究。

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