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首页> 外文期刊>Applied Microbiology >Comparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence
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Comparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence

机译：伯克霍尔德菌洋葱酪氨酸激酶bceF突变体的比较转录组学分析揭示了在对压力，生物膜形成和毒力的耐受性中的作用

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The bacterial tyrosine-kinase (BY-kinase) family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We previously showed that the BceF protein from Burkholderia cepacia IST408 belongs to this BY-kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation of this protein kinase activity. In order to examine this regulation, we performed a comparative transcriptome profile between the bceF mutant and wild-type B. cepacia IST408. The analyses led to identification of 630 genes whose expression was significantly changed. Genes with decreased expression in the bceF mutant were related to stress response, motility, cell adhesion, and carbon and energy metabolism. Genes with increased expression were related to intracellular signaling and lipid metabolism. Mutation of bceF led to reduced survival under heat shock and UV light exposure, reduced swimming motility, and alteration in biofilm architecture when grown in vitro . Consistent with some of these phenotypes, the bceF mutant demonstrated elevated levels of cyclic-di-GMP. Furthermore, BceF contributed to the virulence of B. cepacia for larvae of the Greater wax moth, Galleria mellonella . Taken together, BceF appears to play a considerable role in many cellular processes, including biofilm formation and virulence. As homologues of BceF occur in a number of pathogenic and plant-associated Burkholderia strains, the modulation of bacterial behavior through tyrosine kinase activity is most likely a widely occurring phenomenon.
机译：细菌酪氨酸激酶（BY-激酶）家族包括赋予酪氨酸激酶活性的主要细菌酶。我们先前显示，来自洋葱伯克霍尔德菌IST408的BceF蛋白属于该BY激酶家族，并参与胞外多糖cepacian的生物合成。但是，对该蛋白激酶活性的调节程度了解甚少。为了检查该法规，我们在bceF突变体和野生型洋葱伯克霍尔德菌IST408之间进行了比较转录组分析。该分析导致鉴定了其表达显着改变的630个基因。在bceF突变体中表达降低的基因与应激反应，运动性，细胞粘附以及碳和能量代谢有关。表达增加的基因与细胞内信号转导和脂质代谢有关。在体外生长时，bceF突变导致热休克和紫外线照射下存活率降低，游泳运动降低以及生物膜结构发生改变。与这些表型中的某些一致，bceF突变体表明环二GMP水平升高。此外，BceF增强了洋葱伯克霍尔德逊氏菌对大蜡蛾幼虫（Galleria mellonella）幼虫的毒性。综上所述，BceF似乎在许多细胞过程中起着相当重要的作用，包括生物膜的形成和毒力。由于BceF的同源物出现在许多致病的和植物相关的伯克霍尔德氏菌菌株中，通过酪氨酸激酶活性调节细菌行为很可能是广泛发生的现象。

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