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首页> 外文期刊>Applied Microbiology >Molecular Characterization of Three Lactobacillus delbrueckii subsp. bulgaricus Phages
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Molecular Characterization of Three Lactobacillus delbrueckii subsp. bulgaricus Phages

机译：三种德氏乳杆菌亚种的分子表征。保加利亚噬菌体

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摘要

In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus , named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.
机译：在这项研究中，三个噬菌体感染德氏乳杆菌亚种。从各种工业发酵中得到的乳清样品中分离出了名为Ld3，Ld17和Ld25A的保加利亚。这些噬菌体通过多方面的方法进一步表征：（i）通过宿主范围分析和电子显微镜进行生物学和物理表征; （ii）通过基因组分析进行遗传评估; （iii）对噬菌体的结构成分进行质谱分析; （iv）对于一种噬菌体，通过Northern杂交，逆转录-PCR和引物延伸进行转录分析。获得的三个噬菌体基因组彼此之间以及与所谓的b。L. delbrueckii噬菌体组c5，LL-Ku和phiLdb的基因组之间显示出高度的序列同一性，据信其中观察到的某些差异是宿主的原因范围变化。

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