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首页> 外文期刊>Applied Microbiology >Identification of a Novel Dye-Decolorizing Peroxidase, EfeB, Translocated by a Twin-Arginine Translocation System in Streptococcus thermophilus CGMCC 7.179
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Identification of a Novel Dye-Decolorizing Peroxidase, EfeB, Translocated by a Twin-Arginine Translocation System in Streptococcus thermophilus CGMCC 7.179

机译：通过双精氨酸易位系统在嗜热链球菌CGMCC 7.179中易位的新型染料脱色过氧化物酶EfeB的鉴定。

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Streptococcus thermophilus is a facultative anaerobic bacterium that has the ability to grow and survive in aerobic environments, but the mechanism for this remains unclear. In this study, the efeB gene, encoding a dye-decolorizing peroxidase, was identified in the genome of Streptococcus thermophilus CGMCC 7.179, and purified EfeB was able to decolorize reactive blue 5. Strikingly, genes encoding two components (TatA and TatC) of the twin-arginine translocation (TAT) system were also found in the same operon with the efeB gene. Knocking out efeB or tatC resulted in decreased growth of the strain under aerobic conditions, and complementation of the efeB -deficient strains with the efeB gene enhanced the biomass of the hosts only in the presence of the tatC gene. Moreover, it was proved for both S. thermophilus CGMCC 7.179 and Escherichia coli DE3 that EfeB could be translocated by the TAT system of S. thermophilus . In addition, the transcriptional levels of efeB and tatC increased when the strain was cultured under aerobic conditions. Overall, these results provide the first evidence that EfeB plays a role in protecting cells of S. thermophilus from oxidative stress, with the assistance of the TAT system.
机译：嗜热链球菌是一种兼性厌氧细菌，具有在有氧环境中生长和存活的能力，但其机制尚不清楚。在这项研究中，在嗜热链球菌CGMCC 7.179的基因组中鉴定了编码染料脱色过氧化物酶的efeB基因，并且纯化的EfeB能够使反应性蓝5脱色。在与efeB基因相同的操纵子中也发现了双精氨酸易位（TAT）系统。敲除efeB或tatC导致有氧条件下菌株的生长减少，并且仅在tatC基因存在的情况下，用efeB基因补充efeB缺陷型菌株才能增强宿主的生物量。此外，对于嗜热链球菌CGMCC 7.179和大肠杆菌DE3都证明，EfeB可以被嗜热链球菌的TAT系统转移。另外，当菌株在有氧条件下培养时，efeB和tatC的转录水平增加。总体而言，这些结果提供了第一个证据，证明EfeB在TAT系统的协助下在保护嗜热链球菌细胞免受氧化应激的作用。

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