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首页> 外文期刊>Applied Microbiology >A Survival Strategy for Pseudomonas aeruginosa That Uses Exopolysaccharides To Sequester and Store Iron To Stimulate Psl-Dependent Biofilm Formation
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A Survival Strategy for Pseudomonas aeruginosa That Uses Exopolysaccharides To Sequester and Store Iron To Stimulate Psl-Dependent Biofilm Formation

机译：铜绿假单胞菌的生存策略，使用胞外多糖螯合和存储铁以刺激Psl依赖的生物膜形成。

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Exopolysaccharide Psl is a critical biofilm matrix component in Pseudomonas aeruginosa , which forms a fiber-like matrix to enmesh bacterial communities. Iron is important for P. aeruginosa biofilm development, yet it is not clearly understood how iron contributes to biofilm development. Here, we showed that iron promoted biofilm formation via elevating Psl production in P. aeruginosa . The high level of iron stimulated the synthesis of Psl by reducing rhamnolipid biosynthesis and inhibiting the expression of AmrZ, a repressor of psl genes. Iron-stimulated Psl biosynthesis and biofilm formation held true in mucoid P. aeruginosa strains. Subsequent experiments indicated that iron bound with Psl in vitro and in biofilms, which suggested that Psl fibers functioned as an iron storage channel in P. aeruginosa biofilms. Moreover, among three matrix exopolysaccharides of P. aeruginosa , Psl is the only exopolysaccharide that can bind with both ferrous and ferric ion, yet with higher affinity for ferrous iron. Our data suggest a survival strategy of P. aeruginosa that uses exopolysaccharide to sequester and store iron to stimulate Psl-dependent biofilm formation.IMPORTANCE Pseudomonas aeruginosa is an environmental microorganism which is also an opportunistic pathogen that can cause severe infections in immunocompromised individuals. It is the predominant airway pathogen causing morbidity and mortality in individuals affected by the genetic disease cystic fibrosis (CF). Increased airway iron and biofilm formation have been proposed to be the potential factors involved in the persistence of P. aeruginosa in CF patients. Here, we showed that a high level of iron enhanced the production of the key biofilm matrix exopolysaccharide Psl to stimulate Psl-dependent biofilm formation. Our results not only make the link between biofilm formation and iron concentration in CF, but also could guide the administration or use of iron chelators to interfere with biofilm formation in P. aeruginosa in CF patients. Furthermore, our data also imply a survival strategy of P. aeruginosa under high-iron environmental conditions.
机译：胞外多糖Psl是铜绿假单胞菌中重要的生物膜基质成分，形成纤维状基质以包裹细菌群落。铁对铜绿假单胞菌生物膜的发育很重要，但铁的生物膜发育的作用尚不清楚。在这里，我们表明铁通过提高铜绿假单胞菌的Psl产量促进了生物膜的形成。高铁水平通过减少鼠李糖脂的生物合成和抑制AmrZ的表达来刺激Psl的合成，而AmrZ是psl基因的阻遏物。铁刺激的Psl生物合成和生物膜形成在粘液状铜绿假单胞菌菌株中成立。随后的实验表明，铁在体外和生物膜中均与Psl结合，这表明Psl纤维在铜绿假单胞菌生物膜中起铁存储通道的作用。此外，在铜绿假单胞菌的三种基质胞外多糖中，Psl​​是唯一可以与亚铁离子和铁离子结合的胞外多糖，但对亚铁具有更高的亲和力。我们的数据表明铜绿假单胞菌的生存策略是使用胞外多糖螯合和存储铁以刺激Psl依赖的生物膜形成。重要提示铜绿假单胞菌是一种环境微生物，也是一种机会致病菌，可导致免疫功能低下的人严重感染。它是导致受遗传疾病囊性纤维化（CF）影响的个体发病和死亡的主要呼吸道病原体。有人提议增加气道铁和生物膜形成是CF患者铜绿假单胞菌持续存在的潜在因素。在这里，我们表明，高水平的铁可增强关键生物膜基质胞外多糖Psl的产生，从而刺激Psl依赖性生物膜的形成。我们的结果不仅使生物膜形成与CF中铁浓度之间存在联系，而且还可以指导铁螯合剂的施用或使用来干扰CF患者铜绿假单胞菌生物膜形成。此外，我们的数据还暗示铜绿假单胞菌在高铁环境条件下的生存策略。

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