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首页> 外文期刊>Applied Microbiology >Vancomycin-Resistant Enterococci and Bacterial Community Structure following a Sewage Spill into an Aquatic Environment
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Vancomycin-Resistant Enterococci and Bacterial Community Structure following a Sewage Spill into an Aquatic Environment

机译：污水渗入水生环境后，耐万古霉素的肠球菌和细菌群落结构

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Sewage spills can release antibiotic-resistant bacteria into surface waters, contributing to environmental reservoirs and potentially impacting human health. Vancomycin-resistant enterococci (VRE) are nosocomial pathogens that have been detected in environmental habitats, including soil, water, and beach sands, as well as wildlife feces. However, VRE harboring vanA genes that confer high-level resistance have infrequently been found outside clinical settings in the United States. This study found culturable Enterococcus faecium harboring the vanA gene in water and sediment for up to 3 days after a sewage spill, and the quantitative PCR (qPCR) signal for vanA persisted for an additional week. Culturable levels of enterococci in water exceeded recreational water guidelines for 2 weeks following the spill, declining about five orders of magnitude in sediments and two orders of magnitude in the water column over 6 weeks. Analysis of bacterial taxa via 16S rRNA gene sequencing showed changes in community structure through time following the sewage spill in sediment and water. The spread of opportunistic pathogens harboring high-level vancomycin resistance genes beyond hospitals and into the broader community and associated habitats is a potential threat to public health, requiring further studies that examine the persistence, occurrence, and survival of VRE in different environmental matrices.IMPORTANCE Vancomycin-resistant enterococci (VRE) are harmful bacteria that are resistant to the powerful antibiotic vancomycin, which is used as a last resort against many infections. This study followed the release of VRE in a major sewage spill and their persistence over time. Such events can act as a means of spreading vancomycin-resistant bacteria in the environment, which can eventually impact human health.
机译：污水泄漏可将耐药性细菌释放到地表水中，导致环境蓄水，并可能影响人类健康。耐万古霉素的肠球菌（VRE）是在环境栖息地中检测到的医院病原体，包括土壤，水和沙滩，野生动物的粪便。但是，在美国以外的临床环境中很少发现带有可产生高水平抗药性的vanA基因的VRE。这项研究发现，污水泄漏后长达3天，可培养的粪肠球菌在水中和沉积物中携带有vanA基因，而针对vanA的定量PCR（qPCR）信号又持续了一周。泄漏后的两周内，水中可培养的肠球菌水平超过了娱乐用水准则，在六周内，沉积物中的肠球菌水平下降了约五个数量级，水柱中的下降了两个数量级。通过16S rRNA基因测序对细菌类群进行分析，结果显示随着污水沉积物和水中的溢出，群落结构随时间变化。携带高水平万古霉素抗性基因的机会性病原体在医院外扩散到更广泛的社区和相关栖息地，这是对公共卫生的潜在威胁，需要进一步研究以检查VRE在不同环境基质中的持久性，发生率和存活率。耐万古霉素的肠球菌（VRE）是对强大的抗生素万古霉素具有抗性的有害细菌，万古霉素被用作抵抗许多感染的最后手段。这项研究跟踪了主要污水泄漏中VRE的释​​放及其随时间的持久性。此类事件可作为在环境中传播耐万古霉素细菌的一种手段，这最终会影响人类健康。

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