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首页> 外文期刊>Applied Microbiology >Minimal Growth Temperature, Sodium Chloride Tolerance, pH Sensitivity, and Toxin Production of Marine and Terrestrial Strains of Clostridium botulinum Type C
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Minimal Growth Temperature, Sodium Chloride Tolerance, pH Sensitivity, and Toxin Production of Marine and Terrestrial Strains of Clostridium botulinum Type C

机译：C型肉毒梭菌海洋和陆地菌株的最低生长温度，耐氯化钠耐受性，pH敏感性和毒素产生

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Minimal growth temperatures of four marine and two terrestrial strains of Clostridium botulinum type C were determined in a laboratory culture medium, fortified egg meat medium (FEM), and in ground haddock. The inoculum equaled 2 × 106 viable spores per tube with five-tube replicate sets. The spores were preheated in aqueous suspension at 71 C for 15 min prior to inoculation to reduce toxin carry-over. Similar results were obtained in both substrates. Both the marine and the terrestrial strains grew at 15.6 C, but only the terrestrial strains grew at 12.8 C. None of the strains grew at 10 C during prolonged incubation. The sodium chloride tolerance and the pH sensitivity of the marine and the terrestrial strains were determined at 30 C. The basal medium consisted of beef infusion broth. The inoculum level equaled 2 × 106 unheated spores per replicate. Growth was inhibited at salt concentrations from 2.5 to 3.0%. The terrestrial strains were more pH-sensitive than the marine strains. Whereas the terrestrial strains failed to grow below pH 5.62, three of the marine strains grew at pH 5.10, but not at pH 4.96, during extended incubation. One marine strain grew at pH 5.25, but not below. FEM and proteose peptone-Trypticase-yeast extract-glucose medium permitted the production of high levels of botulinum toxin among four media tested. Toxin produced by the marine and terrestrial strains showed no increase in toxicity after incubation with trypsin.
机译：在实验室培养基，强化蛋肉培养基（FEM）和地下黑线坞中确定了4种海洋和2种C型肉毒梭菌菌株的最低生长温度。接种量等于每管2×106个活孢子，每组5个重复。在接种前将孢子在71°C的水悬浮液中预热15分钟，以减少毒素残留。在两种基材中均获得了相似的结果。海洋和陆地菌株均在15.6 C下生长，但仅陆地菌株在12.8 C下生长。在长时间孵育期间，没有菌株在10 C下生长。在30℃下测定海洋和陆地菌株的氯化钠耐受性和pH敏感性。基础培养基由牛肉浸液组成。每个重复的接种量等于2×106个未加热的孢子。在盐浓度为2.5至3.0％时，生长受到抑制。陆地菌株比海洋菌株对pH更加敏感。尽管陆地菌株无法在pH 5.62以下生长，但其中三株海洋菌株在长时间温育期间的pH为5.10，而不是pH 4.96。一株海洋菌株在pH 5.25处生长，但不能低于pH。有限元分析和蛋白p-胰蛋白tic酶-酵母提取物-葡萄糖培养基可以在四种测试培养基中产生高水平的肉毒杆菌毒素。与胰蛋白酶孵育后，海洋和陆地菌株产生的毒素没有显示出毒性增加。

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