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Increased Radiation Resistance of Vegetative Bacillus pumilus

机译:植物性芽孢杆菌的抗辐射性增加

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A 4.5-fold increase in vegetative cell radiation resistance of Bacillus pumilus E601, the internationally recognized biological standard for irradiation sterilization, was obtained by the repeated passage of resistant survivors through successive sublethal doses of 60Co irradiation. This increase in resistance was accompanied by a corresponding increase in spore resistance through the seventh irradiation passage. By the fifteenth passage, the ability for spore formation was lost. Other effects noted by the successive irradiation dosages included loss of motility and pellicle formation, and changes in the Gram reaction, cell morphology, and colonial morphology. Increased resistance was also accompanied by an increased nutritional requirement for specific amino acids. Radiation resistance was not transferred from vegetative cells to spores.
机译:耐药芽孢杆菌E601(国际公认的辐射灭菌生物标准)的营养细胞辐射抗性提高了4.5倍,这是通过使耐性幸存者反复经过连续的亚致死剂量的60Co辐射而获得的。这种抵抗力的增加伴随着通过第七次照射通道的孢子抵抗力的相应增加。到第十五代时,丧失了形成孢子的能力。连续照射剂量引起的其他影响包括运动力丧失和表皮形成,以及革兰氏反应,细胞形态和菌落形态的变化。抗药性增加还伴随着对特定氨基酸的营养需求增加。辐射抗性没有从营养细胞转移到孢子。

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