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Enumeration and Characterization of Antimicrobial-Resistant Escherichia coli Bacteria in Effluent from Municipal, Hospital, and Secondary Treatment Facility Sources

机译:市政,医院和二级处理设施来源的废水中抗微生物大肠杆菌细菌的计数和表征

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We describe a modification of the most probable number (MPN) method for rapid enumeration of antimicrobial-resistant Escherichia coli bacteria in aqueous environmental samples. E. coli (total and antimicrobial-resistant) bacteria were enumerated in effluent samples from a hospital ( n = 17) and municipal sewers upstream ( n = 5) and downstream ( n = 5) from the hospital, effluent samples from throughout the treatment process ( n = 4), and treated effluent samples ( n = 13). Effluent downstream from the hospital contained a higher proportion of antimicrobial-resistant E. coli than that upstream from the hospital. Wastewater treatment reduced the numbers of E. coli bacteria (total and antimicrobial resistant); however, antimicrobial-resistant E. coli was not eliminated, and E. coli resistant to cefotaxime (including extended-spectrum beta-lactamase [ESBL] producers), ciprofloxacin, and cefoxitin was present in treated effluent samples.
机译:我们描述了一种最可能的数(MPN)方法的修改,用于快速枚举水性环境样品中的抗药性大肠埃希氏菌。从医院(n = 17)和医院下水道(n = 5)和下游(n = 5)的市政污水中抽取大肠杆菌(总耐药菌),在整个处理过程中均进行污水处理处理(n = 4)和处理过的废水样品(n = 13)。医院下游的污水所含的抗药性大肠杆菌比医院上游的高。废水处理减少了大肠埃希菌的数量(总耐药性);但是,未消除对抗生素具有抗药性的大肠杆菌,并且在处理过的污水样品中存在对头孢噻肟(包括广谱β-内酰胺酶[ESBL]生产商),环丙沙星和头孢西丁耐药的大肠杆菌。

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