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Genome and Proteome of Campylobacter jejuni Bacteriophage NCTC 12673

机译：空肠弯曲菌噬菌体NCTC 12673的基因组和蛋白质组

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Campylobacter jejuni continues to be the leading cause of bacterial food-borne illness worldwide, so improvements to current methods used for bacterial detection and disease prevention are needed. We describe here the genome and proteome of C. jejuni bacteriophage NCTC 12673 and the exploitation of its receptor-binding protein for specific bacterial detection. Remarkably, the 135-kb Myoviridae genome of NCTC 12673 differs greatly from any other proteobacterial phage genome described (including C. jejuni phages CP220 and CPt10) and instead shows closest homology to the cyanobacterial T4-related myophages. The phage genome contains 172 putative open reading frames, including 12 homing endonucleases, no visible means of packaging, and a putative trans -splicing intein. The phage DNA appears to be strongly associated with a protein that interfered with PCR amplification and estimation of the phage genome mass by pulsed-field gel electrophoresis. Identification and analyses of the receptor-binding protein (Gp48) revealed features common to the Salmonella enterica P22 phage tailspike protein, including the ability to specifically recognize a host organism. Bacteriophage receptor-binding proteins may offer promising alternatives for use in pathogen detection platforms.
机译：空肠弯曲杆菌仍然是全世界细菌性食源性疾病的主要原因，因此需要对当前用于细菌检测和疾病预防的方法进行改进。我们在这里描述了空肠弯曲杆菌噬菌体NCTC 12673的基因组和蛋白质组，以及其受体结合蛋白用于特定细菌检测的开发。值得注意的是，NCTC 12673的135 kb肌病毒科基因组与所描述的任何其他细菌噬菌体基因组（包括空肠弯曲菌噬菌体CP220和CPt10）有很大不同，而与蓝细菌T4相关的噬菌体具有最接近的同源性。噬菌体基因组包含172个推定的开放阅读框，包括12个归巢核酸内切酶，无可见包装方式和推定的反式内含肽。噬菌体DNA似乎与一种蛋白质紧密结合，该蛋白质干扰PCR扩增和通过脉冲场凝胶电泳估计噬菌体基因组质量。受体结合蛋白（Gp48）的鉴定和分析揭示了沙门氏菌P22噬菌体尾钉蛋白共有的特征，包括特异性识别宿主生物的能力。噬菌体受体结合蛋白可以为病原体检测平台提供有前途的替代方法。

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