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首页> 外文期刊>Applied Microbiology >Transcriptomic Response of Listeria monocytogenes during the Transition to the Long-Term-Survival Phase
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Transcriptomic Response of Listeria monocytogenes during the Transition to the Long-Term-Survival Phase

机译：单核细胞增生李斯特菌向长期生存期过渡的转录组学反应

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Listeria monocytogenes can change its cellular morphology from bacilli to cocci during the transition to the long-term-survival (LTS) phase. The LTS cells demonstrated increased baro- and thermotolerance compared to their vegetative counterparts. So far, the underlying mechanisms that trigger this morphological and physiological transition remain largely unknown. In this study, we compared the transcriptomic profiles of L. monocytogenes serotype 4b strain F2365 at different growth stages in tryptic soy broth with yeast extract (TSBYE) using a whole-genome DNA chip approach. We identified a total of 225 differentially expressed genes (≥4-fold; P < 0.05) during the transition to the LTS phase in TSBYE. Genes related to cell envelope structure, energy metabolism, and transport were most significantly upregulated in the LTS phase. The upregulation of compatible solute transporters may lead to the accumulation of cellular solutes, lowering intracellular water activity and thus increasing bacterial stress resistance during the transition to the LTS phase. The downregulation of genes associated with protein synthesis may indicate a status of metabolic dormancy of the LTS cells. The transcriptomic profiles of resuscitated LTS cells in fresh TSBYE resembled those of log-phase cells ( r =0.94), as the LTS cells rapidly resume metabolic activities and transit back to log phase with decreased baro- and thermotolerance.
机译：单核细胞增生性李斯特菌可以在向长期生存（LTS）阶段过渡期间将其细胞形态从杆菌改变为球菌。 LTS细胞与植物相比具有更高的耐压性和耐热性。到目前为止，触发这种形态和生理转变的潜在机制仍然未知。在这项研究中，我们使用全基因组DNA芯片方法，比较了酵母发酵液（TSBYE）在胰蛋白酶大豆肉汤中不同生长阶段的单核细胞增生李斯特氏菌血清型4b菌株F2365的转录组谱。我们在过渡到LTS阶段的TSBYE中共鉴定了225个差异表达基因（≥4倍; P <0.05）。在LTS阶段，与细胞包膜结构，能量代谢和转运相关的基因被最显着上调。相容性溶质转运蛋白的上调可能导致细胞溶质的积累，降低细胞内水活度，从而在过渡到LTS阶段的过程中增加细菌的抗逆性。与蛋白质合成相关的基因的下调可能表明LTS细胞处于代谢休眠状态。新鲜的TSBYE中复苏的LTS细胞的转录组学特征与对数期细胞相似（r = 0.94），因为LTS细胞迅速恢复新陈代谢活动并过渡到对数期，耐压和耐热性降低。

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