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Aspergillus niger DLFCC-90 Rhamnoside Hydrolase, a New Type of Flavonoid Glycoside Hydrolase

机译:黑曲霉DLFCC-90鼠李糖苷水解酶,一种新型的类黄酮糖苷水解酶

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摘要

A novel rutin-α-l-rhamnosidase hydrolyzing α-l-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
机译:从黑曲霉DLFCC-90中纯化并表征了一种新型水解芦丁,柚皮苷和橙皮苷的α-鼠李糖苷的芦丁-α-鼠李糖苷酶,以及编码该酶的基因,该基因与α-淀粉酶基因高度同源克隆,并在毕赤酵母GS115中表达。该新酶归类为糖苷水解酶(GH)家族13。

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