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首页> 外文期刊>Applied Microbiology >Altered Fecal Microbiota Composition Associated with Food Allergy in Infants
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Altered Fecal Microbiota Composition Associated with Food Allergy in Infants

机译：与婴儿食物过敏相关的粪便微生物群组成改变

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Increasing evidence suggests that perturbations in the intestinal microbiota composition of infants are implicated in the pathogenesis of food allergy (FA), while the actual structure and composition of the intestinal microbiota in human beings with FA remain unclear. Microbial diversity and composition were analyzed with parallel barcoded 454 pyrosequencing targeting the 16S rRNA gene hypervariable V1-V3 regions in the feces of 34 infants with FA (17 IgE mediated and 17 non-IgE mediated) and 45 healthy controls. Here, we showed that several key FA-associated bacterial phylotypes, but not the overall microbiota diversity, significantly changed in infancy fecal microbiota with FA and were associated with the development of FA. The proportion of abundant Bacteroidetes , Proteobacteria , and Actinobacteria phyla were significantly reduced, while the Firmicutes phylum was highly enriched in the FA group ( P < 0.05). Abundant Clostridiaceae 1 organisms were prevalent in infants with FA at the family level ( P = 0.016). FA-enriched phylotypes negatively correlated with interleukin-10, for example, the genera Enterococcus and Staphylococcus . Despite profound interindividual variability, levels of 20 predominant genera were significantly different between the FA and healthy control groups ( P < 0.05). Infants with IgE-mediated FA had increased levels of Clostridium sensu stricto and Anaerobacter and decreased levels of Bacteroides and Clostridium XVIII ( P < 0.05). A positive correlation was observed between Clostridium sensu stricto and serum-specific IgE ( R = 0.655, P < 0.001). The specific microbiota signature could distinguish infants with IgE-mediated FA from non-IgE-mediated ones. Detailed microbiota analysis of a well-characterized cohort of infants with FA showed that dysbiosis of fecal microbiota with several FA-associated key phylotypes may play a pathogenic role in FA.
机译：越来越多的证据表明，婴儿肠道菌群组成的扰动与食物过敏（FA）的发病机理有关，而患有FA的人类肠道菌群的实际结构和组成仍不清楚。用平行条形码454焦磷酸测序对34例FA患儿（17例IgE介导的和17例非IgE介导的）的粪便中的16S rRNA基因高变V1-V3区进行了微生物多样性和组成分析。在这里，我们显示了几种重要的FA相关细菌系统型，但未发现整体微生物群多样性，在FA婴儿粪便微生物群中发生了显着变化，并且与FA的发展有关。 FA组中丰富的拟杆菌，变形杆菌和放线菌门的比例显着降低，而Firmicutes门的高度丰富（P <0.05）。在家庭水平的FA患儿中，丰富的梭菌科1生物普遍存在（P = 0.016）。富含FA的系统型与白介素10呈负相关，例如肠球菌和葡萄球菌属。尽管个体间差异很大，但FA组和健康对照组之间20个主要属的水平显着不同（P <0.05）。由IgE介导的FA的婴儿的严格梭状芽胞杆菌和厌氧菌水平升高，而拟杆菌和梭状芽胞杆菌XVIII的水平降低（P <0.05）。严格梭状芽胞杆菌与血清特异性IgE之间存在正相关（R = 0.655，P <0.001）。特定的微生物群签名可以区分具有IgE介导的FA的婴儿与非IgE介导的FA。对特征明确的FA患儿队列进行的详细微生物群分析表明，粪便微生物群具有几种与FA相关的关键系统型的菌群失调可能在FA中发挥致病作用。

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