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首页> 外文期刊>Applied Microbiology >Dynamic Response of Mycobacterium vanbaalenii PYR-1 to BP Deepwater Horizon Crude Oil
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Dynamic Response of Mycobacterium vanbaalenii PYR-1 to BP Deepwater Horizon Crude Oil

机译：Vanbaalenii分枝杆菌PYR-1对BP深水地平线原油的动态响应

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We investigated the response of the hydrocarbon-degrading Mycobacterium vanbaalenii PYR-1 to crude oil from the BP Deepwater Horizon (DWH) spill, using substrate depletion, genomic, and proteome analyses. M. vanbaalenii PYR-1 cultures were incubated with BP DWH crude oil, and proteomes and degradation of alkanes and polycyclic aromatic hydrocarbons (PAHs) were analyzed at four time points over 30 days. Gas chromatography-mass spectrometry (GC-MS) analysis showed a chain length-dependent pattern of alkane degradation, with C_(12) and C_(13) being degraded at the highest rate, although alkanes up to C_(28) were degraded. Whereas phenanthrene and pyrene were completely degraded, a significantly smaller amount of fluoranthene was degraded. Proteome analysis identified 3,948 proteins, with 876 and 1,859 proteins up- and downregulated, respectively. We observed dynamic changes in protein expression during BP crude oil incubation, including transcriptional factors and transporters potentially involved in adaptation to crude oil. The proteome also provided a molecular basis for the metabolism of the aliphatic and aromatic hydrocarbon components in the BP DWH crude oil, which included upregulation of AlkB alkane hydroxylase and an expression pattern of PAH-metabolizing enzymes different from those in previous proteome expression studies of strain PYR-1 incubated with pure or mixed PAHs, particularly the ring-hydroxylating oxygenase (RHO) responsible for the initial oxidation of aromatic hydrocarbons. Based on these results, a comprehensive cellular response of M. vanbaalenii PYR-1 to BP crude oil was proposed. This study increases our fundamental understanding of the impact of crude oil on the cellular response of bacteria and provides data needed for development of practical bioremediation applications.
机译：我们使用底物耗竭，基因组和蛋白质组分析研究了降解石油的分枝杆菌分枝杆菌PYR-1对来自BP深水地平线（DWH）溢油的原油的反应。用BP DWH原油孵育M. vanbaalenii PYR-1培养物，并在30天的四个时间点分析蛋白质组以及烷烃和多环芳烃（PAH）的降解。气相色谱-质谱（GC-MS）分析显示链长依赖链烷烃的降解模式，尽管高达C_（28）的烷烃被降解，但C_（12）和C_（13）的降解速率最高。菲和pyr被完全降解，而荧蒽的数量却大大减少。蛋白质组分析确定了3,948个蛋白质，其中分别有876和1859个蛋白质被上调和下调。我们观察到了BP原油孵育过程中蛋白质表达的动态变化，包括转录因子和潜在参与原油适应的转运蛋白。蛋白质组学还为BP DWH原油中脂族和芳族烃组分的代谢提供了分子基础，其中包括AlkB烷烃羟化酶的上调和PAH代谢酶的表达模式与以前的菌株蛋白质组表达研究不同。将PYR-1与纯净或混合的PAHs孵育，尤其是负责芳烃初始氧化的环羟基化加氧酶（RHO）。基于这些结果，提出了南美白对虾PYR-1对BP原油的全面细胞应答。这项研究增加了我们对原油对细菌细胞反应的影响的基本了解，并提供了开发实际生物修复应用所需的数据。

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