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Evidence of Apis ceranaSacbrood virus Infection in Apis mellifera

机译：蜜蜂在蜜蜂中的证据

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Sacbrood virus (SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera . In previous studies, SBV isolates infecting A. cerana (AcSBV) and SBV isolates infecting A. mellifera (AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318 A. mellifera colonies and 64 A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38 A. mellifera colonies and 37 A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated from A. mellifera were clustered with the A. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae of A. mellifera , although there were no obvious signs of the disease, demonstrating the replication of AcSBV in A. mellifera . Our results suggest that AcSBV is able to infect A. mellifera colonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities of A. cerana and A. mellifera to sacbrood disease and is potentially useful for guiding beekeeping practices.
机译：Sacbrood病毒（SBV）是亚洲蜜蜂Apis cerana中最具破坏性的病毒之一，但是对Apis mellifera的破坏性要小得多。在以前的研究中，感染了中华曲霉的SBV分离株（AcSBV）和感染了苜蓿曲霉（AmSBV）的SBV分离株被鉴定为不同的血清型，表明在SBV感染中存在物种屏障。为了调查这种物种分离，我们检查了318 A. mellifera菌落和64 A. cerana菌落中SBV感染的存在，并鉴定了SBV分离株的基因型。我们还在实验室和野外条件下进行了人工感染实验。结果显示，38 mellifera菌落和37 cerana cerana菌落均为SBV感染阳性。基于RNA依赖性RNA聚合酶（RdRp）基因序列的系统发育分析表明，中华曲霉分离株和大多数拟南芥分离株形成两个不同的进化枝，但从拟南芥分离的两个菌株与中华曲霉分离。在人工感染实验中，虽然没有明显迹象表明该病的迹象，但在蜜蜂和成虫的幼虫中都可以检测到AcSBV负链RNA，这证明了AcSBV在蜜蜂中的复制。我们的结果表明，AcSBV能够以低患病率（本研究中为0.63％）和致病性感染A.mellifera菌落。这项工作将有助于解释cerana cerana和mellifera对sacbrood疾病的不同敏感性，并可能对指导养蜂实践有用。

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