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首页> 外文期刊>Applied Microbiology >Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments
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Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments

机译：从法国沿海环境分离的产志贺毒素的大肠杆菌和肠致病性大肠杆菌的分子谱分析

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Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin ( stx ) and intimin ( eae ) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large number or a particular combination of genetic markers of virulence and the presence of stx and/or eae variants, suggesting their potential pathogenicity for humans. Among these, an O26:H11 stx _(1a) eae -β1 strain was associated with a large number of virulence-associated genes ( n = 47), including genes carried on the locus of enterocyte effacement (LEE) or other pathogenicity islands, such as OI-122, OI-71, OI-43/48, OI-50, OI-57, and the high-pathogenicity island (HPI). One O91:H21 STEC strain containing 4 stx variants ( stx _(1a), stx _(2a), stx _(2c), and stx _(2d)) was found to possess genes associated with pathogenicity islands OI-122, OI-43/48, and OI-15. Among EPEC strains harboring a large number of virulence genes ( n , 34 to 50), eight belonged to serotype O26:H11, O103:H2, O103:H25, O145:H28, O157:H7, or O153:H2.IMPORTANCE The species E. coli includes a wide variety of strains, some of which may be responsible for severe infections. This study, a molecular risk assessment study of E. coli strains isolated from the coastal environment, was conducted to evaluate the potential risk for shellfish consumers. This report describes the characterization of virulence gene profiles and stx / eae polymorphisms of E. coli isolates and clearly highlights the finding that the majority of strains isolated from coastal environment are potentially weakly pathogenic, while some are likely to be more pathogenic.
机译：产生志贺毒素的大肠杆菌（STEC）和肠致病性大肠杆菌（EPEC）菌株可能是人类食源性感染的原因。这项研究的特征是先前从法国贝类收获地区及其流域分离的28种STEC和75种EPEC菌株，它们属于68种可区分的血清型。使用高通量实时PCR来搜索75个与大肠杆菌毒性相关的基因靶标，并分别使用PCR试验和DNA测序对编码志贺毒素（stx）和内膜素（eae）的基因进行亚型分析。结果表明，菌株之间具有高度的多样性，STEC具有17种独特的毒力基因谱，EPEC具有56种独特的毒力基因谱。发现7株STEC和15株EPEC菌株显示出大量或特定的毒力遗传标志物以及stx和/或eae变体的组合，表明它们对人类具有潜在的致病性。其中，O26：H11 stx _（1a）eae-β1菌株与大量毒力相关基因（n = 47）相关，包括在肠上皮细胞受损（LEE）或其他致病岛上携带的基因，例如OI-122，OI-71，OI-43 / 48，OI-50，OI-57和高致病性岛（HPI）。发现一种O91：H21 STEC菌株包含4种stx变体（stx _（1a），stx _（2a），stx _（2c）和stx _（2d））具有与致病岛OI-122，OI相关的基因-43/48和OI-15。在具有大量毒力基因（n，34至50）的EPEC菌株中，有8个属于O26：H11，O103：H2，O103：H25，O145：H28，O157：H7或O153：H2血清型。大肠杆菌包括各种各样的菌株，其中一些可能是造成严重感染的原因。这项研究是对从沿海环境中分离出的大肠杆菌进行的分子风险评估研究，旨在评估贝类消费者的潜在风险。该报告描述了大肠杆菌分离物的毒力基因概况和stx / eae多态性的特征，并明确强调了以下发现：从沿海环境中分离出的大多数菌株具有潜在的弱致病性，而另一些则可能具有更高的致病性。

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