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首页> 外文期刊>Applied Microbiology >Improved Electrotransformation and Decreased Antibiotic Resistance of the Cystic Fibrosis Pathogen Burkholderia cenocepacia Strain J2315
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Improved Electrotransformation and Decreased Antibiotic Resistance of the Cystic Fibrosis Pathogen Burkholderia cenocepacia Strain J2315

机译：囊性纤维化病原菌伯克霍尔德森新生菌菌株J2315的改善的电转化和降低的抗生素耐药性

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The bacterium Burkholderia cenocepacia is pathogenic for sufferers from cystic fibrosis (CF) and certain immunocompromised conditions. The B. cenocepacia strain most frequently isolated from CF patients, and which serves as the reference for CF epidemiology, is J2315. The J2315 genome is split into three chromosomes and one plasmid. The strain was sequenced several years ago, and its annotation has been released recently. This information should allow genetic experimentation with J2315, but two major impediments appear: the poor potential of J2315 to act as a recipient in transformation and conjugation and the high level of resistance it mounts to nearly all antibiotics. Here, we describe modifications to the standard electroporation procedure that allow routine transformation of J2315 by DNA. In addition, we show that deletion of an efflux pump gene and addition of spermine to the medium enhance the sensitivity of J2315 to certain commonly used antibiotics and so allow a wider range of antibiotic resistance genes to be used for selection.
机译：伯克霍尔德氏菌细菌是致囊性纤维化（CF）和某些免疫功能低下患者的致病菌。从CF患者中最常分离到的B. cenocepacia菌株是J2315，它是CF流行病学的参考。 J2315基因组被分为三个染色体和一个质粒。该菌株于几年前进行了测序，其注释已于近期发布。该信息应允许对J2315进行基因实验，但出现两个主要障碍：J2315在转化和结合过程中作为受体的潜在能力较弱，并且几乎对所有抗生素都具有较高的抗药性。在这里，我们描述了对标准电穿孔程序的修改，该修改允许通过DNA常规转化J2315。另外，我们显示出外排泵基因的缺失和向培养基中添加精胺提高了J2315对某些常用抗生素的敏感性，因此可以选择更广泛的抗生素抗性基因。

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