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Genetic Analysis for the Lack of Expression of the O157 Antigen in an O Rough:H7 Escherichia coli Strain

机译:O粗糙:H7大肠杆菌菌株中O157抗原缺乏表达的遗传分析

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摘要

The O-antigen ( rfb ) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS 629 , was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6.
机译:检查了O6粗糙:H7志贺毒素大肠杆菌菌株MA6的O抗原(rfb)操纵子和相关基因,以确定O157表达缺乏的原因。在其gne基因内观察到与IS 629同源的1,310 bp插入。与来自O157:H7的功能性gne基因进行反式互补可恢复MA6中O157抗原的表达。

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