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首页> 外文期刊>Applied Microbiology >Autonomous Growth of Isolated Single Listeria monocytogenes and Salmonella enterica Serovar Typhimurium Cells in the Absence of Growth Factors and Intercellular Contact
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Autonomous Growth of Isolated Single Listeria monocytogenes and Salmonella enterica Serovar Typhimurium Cells in the Absence of Growth Factors and Intercellular Contact

机译：在没有生长因子和细胞间接触的情况下分离的单核细胞增生李斯特菌和肠炎沙门氏菌鼠伤寒沙门氏菌细胞的自主生长。

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The aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. In order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. This was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. Over 450 single isolated Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Typhimurium cells in lag, log, and stationary growth phases were investigated by this method, which included thoroughly washing the cells. The proportion of living cells within the initial cultures was compared to the proportion of positive samples after enrichment of the separated single cells. This resulted in P values of ≥0.05 using the chi-square test for statistical analysis, indicating no significant difference, and clearly demonstrates reproduction of isolated single bacterial cells without the need for growth factors or intercellular contact. Ease of handling of the apparatus and good performance of the cleaning procedures were achieved, as was validation of the method, demonstrating its suitability for routine laboratory use.
机译：这项研究的目的是在没有生长因子和细胞间接触的情况下观察分离的单个细菌细胞的生长。为了排除稀释系列引起的随机不确定性，开发了一种新的显微操作方法，以确保在视觉控制下获得明确的结果。进行此操作时要特别小心，以生产单个原核细胞并随后对其自主生长进行研究。超过450种分离的单核细胞增生李斯特菌和肠炎沙门氏菌亚种。通过这种方法研究了在滞后，对数和静止生长期的肠型血清鼠伤寒沙门氏菌细胞，包括彻底清洗细胞。富集分离的单细胞后，将初始培养物中活细胞的比例与阳性样品的比例进行比较。使用卡方检验进行统计分析得出的P值≥0.05，表明无显着差异，并清楚地证明了分离的单个细菌细胞的繁殖，而无需生长因子或细胞间接触。简便易用的设备以及良好的清洁程序性能，以及方法的验证，证明了其适合常规实验室使用。

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