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首页> 外文期刊>Applied Microbiology >Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in Escherichia coli Strains Engineered for the Production of Ethanol and Lactate
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Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in Escherichia coli Strains Engineered for the Production of Ethanol and Lactate

机译：由于NADH依赖的氧化还原酶FucO过表达在设计用于生产乙醇和乳酸的大肠杆菌中的糠醛耐受性增加

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摘要

Furfural is an important fermentation inhibitor in hemicellulose sugar syrups derived from woody biomass. The metabolism of furfural by NADPH-dependent oxidoreductases, such as YqhD (low K_(m) for NADPH), is proposed to inhibit the growth and fermentation of xylose in Escherichia coli by competing with biosynthesis for NADPH. The discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural provided a new approach to improve furfural tolerance. Strains that produced ethanol or lactate efficiently as primary products from xylose were developed. These strains included chromosomal mutations in yqhD expression that permitted the fermentation of xylose broths containing up to 10 mM furfural. Expression of fucO from plasmids was shown to increase furfural tolerance by 50% and to permit the fermentation of 15 mM furfural. Product yields with 15 mM furfural were equivalent to those of control strains without added furfural (85% to 90% of the theoretical maximum). These two defined genetic traits can be readily transferred to enteric biocatalysts designed to produce other products. A similar strategy that minimizes the depletion of NADPH pools by native detoxification enzymes may be generally useful for other inhibitory compounds in lignocellulosic sugar streams and with other organisms.
机译：糠醛是木质生物质衍生的半纤维素糖浆中的重要发酵抑制剂。有人提出通过NADPH依赖的氧化还原酶（例如YqhD（NADPH的K_（m）低））代谢糠醛，通过与NADPH的生物合成竞争来抑制木糖在大肠杆菌中的生长和发酵。 NADH依赖的丙二醇氧化还原酶（FucO）可以减少糠醛的发现为提高糠醛耐受性提供了一种新方法。已开发出可从木糖中高效生产乙醇或乳酸作为主要产物的菌株。这些菌株在yqhD表达中包含染色体突变，从而允许发酵含10 mM糠醛的木糖肉汤。显示从质粒表达fucO可将糠醛耐受性提高50％，并允许发酵15 mM糠醛。糠醛含量为15 mM的产品产量与不添加糠醛的对照菌株的产量相同（理论最大值的85％至90％）。这两个定义的遗传特征可以很容易地转移到设计用于生产其他产品的肠溶生物催化剂中。使木质素解毒酶对NADPH库的消耗最小化的类似策略通常可用于木质纤维素糖流中的其他抑制性化合物以及其他生物。

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