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首页> 外文期刊>Applied Microbiology >Evolution of Secondary Metabolite Genes in Three Closely Related Marine Actinomycete Species
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Evolution of Secondary Metabolite Genes in Three Closely Related Marine Actinomycete Species

机译：三种密切相关的海洋放线菌物种中次级代谢产物基因的进化

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The marine actinomycete genus Salinispora is composed of three closely related species. These bacteria are a rich source of secondary metabolites, which are produced in species-specific patterns. This study examines the distribution and phylogenetic relationships of genes involved in the biosynthesis of secondary metabolites in the salinosporamide and staurosporine classes, which have been reported for S. tropica and S. arenicola , respectively. The focus is on “ Salinispora pacifica ,” the most recently discovered and phylogenetically diverse member of the genus. Of 61 S. pacifica strains examined, 15 tested positive for a ketosynthase (KS) domain linked to the biosynthesis of salinosporamide K, a new compound in the salinosporamide series. Compound production was confirmed in two strains, and the domain phylogeny supports vertical inheritance from a common ancestor shared with S. tropica , which produces related compounds in the salinosporamide series. There was no evidence for interspecies recombination among salA KS sequences, providing further support for the geographic isolation of these two salinosporamide-producing lineages. In addition, staurosporine production is reported for the first time for S. pacifica , with 24 of 61 strains testing positive for staD , a key gene involved in the biosynthesis of this compound. High levels of recombination were observed between staD alleles in S. pacifica and the cooccurring yet more distantly related S. arenicola , which produces a similar series of staurosporines. The distributions and phylogenies of the biosynthetic genes examined provide insight into the complex processes driving the evolution of secondary metabolism among closely related bacterial species.
机译：海洋放线菌属Salinispora由三个密切相关的物种组成。这些细菌是次级代谢产物的丰富来源，这些代谢产物以物种特异性模式产生。这项研究检查了盐生孢子酰胺和星形孢菌素类中次生代谢产物的生物合成相关基因的分布和系统发育关系，分别报道了热带链球菌和沙门氏菌。重点是“ Salinispora pacifica”（Salinispora pacifica），它是该属中最近发现的，系统发育上多样的成员。在所检查的61个太平洋链球菌菌株中，有15个测试的酮合酶（KS）结构域与salinosporamide K（salinosporamide系列中的新化合物）的生物合成有关，呈阳性。在两个菌株中证实了化合物的产生，并且系统发育树支持与热带假单胞菌共有的共同祖先的垂直遗传，热带假单胞菌产生了盐孢子酰胺系列中的相关化合物。没有证据表明salA KS序列之间存在种间重组，这为这两个产生salinosporamide的谱系的地理隔离提供了进一步的支持。此外，首次报道了葡萄球菌产生星形孢菌素，在61个菌株中有24个对staD（该化合物生物合成中涉及的关键基因）测试呈阳性。在太平洋链球菌中的staD等位基因与发生距离更远的亲缘链球菌之间发生了高水平的重组，产生了一系列类似的星形孢菌素。被检查的生物合成基因的分布和系统发育提供了深入洞察驱动密切相关细菌物种间次级代谢演变的复杂过程的信息。

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