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首页> 外文期刊>Applied Microbiology >Inactivation of Escherichia coli by Polychromatic Simulated Sunlight: Evidence for and Implications of a Fenton Mechanism Involving Iron, Hydrogen Peroxide, and Superoxide
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Inactivation of Escherichia coli by Polychromatic Simulated Sunlight: Evidence for and Implications of a Fenton Mechanism Involving Iron, Hydrogen Peroxide, and Superoxide

机译：多色模拟阳光灭活大肠杆菌：涉及铁，过氧化氢和超氧化物的Fenton机制的证据及其意义

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摘要

Sunlight inactivation of Escherichia coli has previously been shown to accelerate in the presence of oxygen, exogenously added hydrogen peroxide, and bioavailable forms of exogenously added iron. In this study, mutants unable to effectively scavenge hydrogen peroxide or superoxide were found to be more sensitive to polychromatic simulated sunlight (without UVB wavelengths) than wild-type cells, while wild-type cells grown under low-iron conditions were less sensitive than cells grown in the presence of abundant iron. Furthermore, prior exposure to simulated sunlight was found to sensitize cells to subsequent hydrogen peroxide exposure in the dark, but this effect was attenuated for cells grown with low iron. Mutants deficient in recombination DNA repair were sensitized to simulated sunlight (without UVB wavelengths), but growth in the presence of iron chelators reduced the degree of sensitization conferred by this mutation. These findings support the hypothesis that hydrogen peroxide, superoxide, and intracellular iron all participate in the photoinactivation of E. coli and further suggest that the inactivation rate of enteric bacteria in the environment may be strongly dependent on iron availability and growth conditions.
机译：先前已经证明，在氧气，外源添加的过氧化氢和外源添加的铁的生物可利用形式的存在下，大肠杆菌的日光灭活会加速。在这项研究中，发现不能有效清除过氧化氢或超氧化物的突变体比野生型细胞对多色模拟阳光（无UVB波长）更敏感，而在低铁条件下生长的野生型细胞则比野生型细胞敏感。在丰富的铁存在下生长。此外，发现先前暴露于模拟阳光下会使细胞对随后在黑暗中的过氧化氢暴露敏感，但是对于低铁生长的细胞，这种作用减弱了。缺乏重组DNA修复的突变体对模拟的阳光（无UVB波长）敏感，但在铁螯合剂存在下生长会降低这种突变所引起的敏感度。这些发现支持以下假设：过氧化氢，超氧化物和细胞内铁均参与大肠杆菌的光灭活，并进一步表明环境中肠细菌的失活率可能强烈取决于铁的有效性和生长条件。

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