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首页> 外文期刊>Applied Microbiology >De Novo Amino Acid Biosynthesis Contributes to Salmonella enterica Growth in Alfalfa Seedling Exudates
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De Novo Amino Acid Biosynthesis Contributes to Salmonella enterica Growth in Alfalfa Seedling Exudates

机译：De Novo氨基酸的生物合成有助于苜蓿幼苗分泌物中肠炎沙门氏菌的生长

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Salmonella enterica is a member of the plant microbiome. Growth of S. enterica in sprouting-seed exudates is rapid; however, the active metabolic networks essential in this environment are unknown. To examine the metabolic requirements of S. enterica during growth in sprouting-seed exudates, we inoculated alfalfa seeds and identified 305 S. enterica proteins extracted 24 h postinoculation from planktonic cells. Over half the proteins had known metabolic functions, and they are involved in over one-quarter of the known metabolic reactions. Ion and metabolite transport accounted for the majority of detected reactions. Proteins involved in amino acid transport and metabolism were highly represented, suggesting that amino acid metabolic networks may be important for S. enterica growth in association with roots. Amino acid auxotroph growth phenotypes agreed with the proteomic data; auxotrophs in amino acid-biosynthetic pathways that were detected in our screen developed growth defects by 48 h. When the perceived sufficiency of each amino acid was expressed as a ratio of the calculated biomass requirement to the available concentration and compared to growth of each amino acid auxotroph, a correlation between nutrient availability and bacterial growth was found. Furthermore, glutamate transport acted as a fitness factor during S. enterica growth in association with roots. Collectively, these data suggest that S. enterica metabolism is robust in the germinating-alfalfa environment; that single-amino-acid metabolic pathways are important but not essential; and that targeting central metabolic networks, rather than dedicated pathways, may be necessary to achieve dramatic impacts on bacterial growth.
机译：肠沙门氏菌是植物微生物组的成员。肠炎链球菌在发芽种子分泌物中的生长迅速;然而，在这种环境下必需的活性代谢网络尚不清楚。为了检查在发芽种子渗出液中生长过程中肠炎沙门氏菌的代谢需求，我们接种了苜蓿种子，并鉴定了接种后24小时从浮游细胞中提取的305种肠沙门氏菌蛋白质。超过一半的蛋白质具有已知的代谢功能，它们参与了超过四分之一的已知代谢反应。离子和代谢物运输占检测到的大多数反应。参与氨基酸转运和代谢的蛋白质被高度代表，表明氨基酸代谢网络对于与根相关的小肠链球菌生长可能很重要。氨基酸营养缺陷型生长表型与蛋白质组学数据相符。在我们的筛选中检测到的氨基酸生物合成途径中的营养缺陷型细菌在48 h内出现了生长缺陷。当将每种氨基酸的感知充足性表示为计算出的生物量需求与有效浓度的比率，并将其与每种氨基酸营养缺陷型的生长进行比较时，发现养分利用率与细菌生长之间存在相关性。此外，谷氨酸转运与根系一起在肠炎沙门氏菌生长过程中作为健身因子。总体而言，这些数据表明，肠沙门氏菌在发芽苜蓿环境中的代谢很强。单氨基酸代谢途径很重要但不是必需的;并且可能需要针对中央代谢网络而不是专用途径，以实现对细菌生长的显着影响。

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