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首页> 外文期刊>Applied Microbiology >Pseudofructophilic Leuconostoc citreum Strain F192-5, Isolated from Satsuma Mandarin Peel
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Pseudofructophilic Leuconostoc citreum Strain F192-5, Isolated from Satsuma Mandarin Peel

机译：从萨摩柑桔皮中分离出的拟果胶柠檬酸亮葡菌F192-5菌株

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Fructophilic lactic acid bacteria (FLAB), composed of Fructobacillus spp., Lactobacillus kunkeei, and Lactobacillus apinorum, are unique in that they prefer d-fructose over d-glucose as a carbon source. Strain F192-5, isolated from the peel of a satsuma mandarin and identified as Leuconostoc citreum, grows well on d-fructose but poorly on d-glucose and produces mainly lactate and acetate, with trace amounts of ethanol, from the metabolism of d-glucose. These characteristics are identical to those of obligate FLAB. However, strain F192-5 ferments a greater variety of carbohydrates than known FLAB. Comparative analyses of the genomes of strain F192-5 and reference strains of L. citreum revealed no signs of specific gene reductions, especially genes involved in carbohydrate transport and metabolism, in the genome of F192-5. The bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) is conserved in strain F192-5 but is not transcribed. This is most likely due to a deletion in the promoter region upstream of the adhE gene. Strain F192-5 did, however, ferment d-glucose when transformed with a plasmid containing the allochthonous adhE gene. L. citreum F192-5 is an example of a pseudo-FLAB strain with a deficiency in d-glucose metabolism. This unique phenotypic characteristic appears to be strain specific within the species L. citreum. This might be one of the strategies lactic acid bacteria use to adapt to diverse environmental conditions.IMPORTANCE Obligate fructophilic lactic acid bacteria (FLAB) lack the metabolic pathways used in the metabolism of most carbohydrates and differ from other lactic acid bacteria in that they prefer to ferment d-fructose instead of d-glucose. These characteristics are well conserved at the genus or species level. Leuconostoc citreum F192-5 shows similar growth characteristics. However, the strain is metabolically and genomically different from obligate FLAB. This is an example of a strain that evolved a pseudo-FLAB phenotype to adapt to a fructose-rich environment.
机译：果糖乳酸菌（FLAB）由果糖杆菌属，kunkeei乳杆菌和apinorum乳杆菌组成，其独特之处在于它们比d-葡萄糖更喜欢d-果糖作为碳源。从萨萨玛柑的果皮中分离出来的F192-5菌株被鉴定为柠檬亮褐，在d-果糖上生长良好，但在d-葡萄糖上生长较差，主要通过d-的代谢产生乳酸和乙酸，以及少量的乙醇。葡萄糖。这些特性与专用FLAB相同。但是，与已知的FLAB相比，F192-5菌株发酵的碳水化合物种类更多。对菌株F192-5和参考菌株柠檬酸乳杆菌的基因组的比较分析显示，在F192-5的基因组中没有特异性基因减少的迹象，特别是涉及碳水化合物转运和代谢的基因。双功能醇/乙醛脱氢酶基因（adhE）在F192-5菌株中保守，但未转录。这很可能是由于adhE基因上游启动子区域的缺失。然而，当用含有异源adhE基因的质粒转化时，菌株F192-5确实发酵了d-葡萄糖。柠檬乳杆菌F192-5是d-葡萄糖代谢缺乏的假FLAB菌株的实例。这种独特的表型特征似乎是柠檬酸乳杆菌中的菌株特异性。这可能是乳酸菌适应各种环境条件的策略之一。要点专性果糖乳酸菌（FLAB）缺乏大多数碳水化合物代谢所使用的代谢途径，并且与其他乳酸菌不同，它们更喜欢发酵d-果糖而不是d-葡萄糖。这些特征在属或种水平上都得到很好的保守。柠檬色隐球菌F192-5具有相似的生长特性。但是，该菌株在代谢和基因组上与专制FLAB不同。这是进化出伪FLAB表型以适应富含果糖的环境的菌株的实例。

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