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Rapid Method for Isolation of Desiccation-Tolerant Strains and Xeroprotectants

机译：干燥耐性菌株和干保护剂的快速分离方法

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A novel biotechnological process has been developed for the isolation of desiccation-tolerant microorganisms and their xeroprotectants, i.e., compatible solutes involved in long-term stability of biomolecules in the dry state. Following exposure of soil samples to chloroform, we isolated a collection of desiccation-tolerant microorganisms. This collection was screened for the production of xeroprotectants by a variation of the bacterial milking (osmotic downshock) procedure and by a novel air-drying/rehydration (“dry milking”) incubation method. The resultant solutes were shown to protect both proteins and living cells against desiccation damage, thereby validating them as xeroprotectants. Nuclear magnetic resonance (NMR) analytical studies were performed to identify the xeroprotectants; synthetic mixtures of these compounds were shown to perform similarly to natural isolates in drying experiments with proteins and cells. This new approach has biotechnological and environmental implications for the identification of new xeroprotectants of commercial and therapeutic value.
机译：已经开发出一种新的生物技术方法，用于分离耐干燥的微生物及其耐干剂，即，参与干燥状态的生物分子长期稳定性的相容性溶质。将土壤样品暴露于氯仿后，我们分离出了一组耐干燥性的微生物。通过改变细菌挤奶（渗透性向下冲击）程序和通过新颖的风干/补液（“干挤奶”）温育方法来筛选该集合物的干保护剂的产生。结果表明，所形成的溶质可保护蛋白质和活细胞免受干燥损伤，从而将其验证为干保护剂。进行了核磁共振（NMR）分析研究以鉴定干保护剂。这些化合物的合成混合物在蛋白质和细胞的干燥实验中显示出与天然分离物相似的性能。这种新方法对于鉴定具有商业和治疗价值的新型干防护剂具有生物技术和环境意义。

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