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首页> 外文期刊>Applied Microbiology >Classification of Lytic Bacteriophages Attacking Dairy Leuconostoc Starter Strains
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Classification of Lytic Bacteriophages Attacking Dairy Leuconostoc Starter Strains

机译：攻击乳白乳初生菌的溶菌噬菌体的分类

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A set of 83 lytic dairy bacteriophages (phages) infecting flavor-producing mesophilic starter strains of the Leuconostoc genus was characterized, and the first in-depth taxonomic scheme was established for this phage group. Phages were obtained from different sources, i.e., from dairy samples originating from 11 German dairies (50 Leuconostoc pseudomesenteroides [ Ln. pseudomesenteroides ] phages, 4 Ln. mesenteroides phages) and from 3 external phage collections (17 Ln. pseudomesenteroides phages, 12 Ln. mesenteroides phages). All phages belonged to the Siphoviridae family of phages with isometric heads (diameter, 55 nm) and noncontractile tails (length, 140 nm). With the exception of one phage (i.e., phage ΦLN25), all Ln. mesenteroides phages lysed the same host strains and revealed characteristic globular baseplate appendages. Phage ΦLN25, with different Y-shaped appendages, had a unique host range. Apart from two phages (i.e., phages P792 and P793), all Ln. pseudomesenteroides phages shared the same host range and had plain baseplates without distinguishable appendages. They were further characterized by the presence or absence of a collar below the phage head or by unique tails with straight striations. Phages P792 and P793 with characteristic fluffy baseplate appendages could propagate only on other specific hosts. All Ln. mesenteroides and all Ln. pseudomesenteroides phages were members of two (host species-specific) distinct genotypes but shared a limited conserved DNA region specifying their structural genes. A PCR detection system was established and was shown to be reliable for the detection of all Leuconostoc phage types.
机译：表征了83个溶菌性乳杆菌噬菌体（噬菌体），它们感染了Leuconostoc属的产生香味的嗜温发酵菌，并为该噬菌体组建立了第一个深入的分类方案。噬菌体是从不同来源获得的，即来自11个德国奶牛场的乳制品样品（50个Leuconostoc pseudomesenteroides [Ln。pseudomesenteroides]噬菌体，4 L. mesenteroides噬菌体）和3个外部噬菌体集合（17 Ln。pseudomesenteroides噬菌体，12 Ln）。 mesenteroides噬菌体）。所有噬菌体均属于Siphoviridae噬菌体家族，具有等长的头部（直径55 nm）和不收缩的尾巴（长度140 nm）。除了一个噬菌体（即噬菌体ΦLN25）外，所有Ln。 Mesenteroides噬菌体裂解了相同的宿主菌株，并揭示了特征性的球形底板附件。具有不同Y形附件的噬菌体ΦLN25具有独特的宿主范围。除了两个噬菌体（即噬菌体P792和P793）外，所有Ln都为Ln。假性肠膜沙门氏菌噬菌体具有相同的宿主范围，并且具有纯净的底板，没有可分辨的附肢。它们的进一步特征是在噬菌体头下方是否存在项圈或具有直条纹的独特尾巴。具有特征性蓬松底板附件的噬菌体P792和P793仅可在其他特定宿主上传播。全部Ln。 mesenteroides和所有Ln。假性肠膜肠炎噬菌体是两种（特定于宿主物种的）不同基因型的成员，但共有一个有限的保守DNA区域，指定了它们的结构基因。建立了一个PCR检测系统，该系统显示出可用于检测所有Leuconostoc噬菌体类型。

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