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首页> 外文期刊>Applied Microbiology >Thermal and Nonthermal Effects of Discontinuous Microwave Exposure (2.45 Gigahertz) on the Cell Membrane of Escherichia coli
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Thermal and Nonthermal Effects of Discontinuous Microwave Exposure (2.45 Gigahertz) on the Cell Membrane of Escherichia coli

机译：不连续微波照射（2.45吉赫兹）对大肠杆菌细胞膜的热和非热效应

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摘要

The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW exposure. To this purpose, microwave irradiation of bacteria was performed under carefully defined and controlled parameters, resulting in a discontinuous MW exposure in order to maintain the average temperature of the bacterial cell suspensions at 37°C. Escherichia coli cells were exposed to 200- to 2,000-W discontinuous microwave (DW) treatments for different periods of time. For each experiment, conventional heating (CH) in a water bath at 37°C was performed as a control. The effects of DW exposure on cell membranes was investigated using flow cytometry (FCM), after propidium iodide (PI) staining of cells, in addition to the assessment of intracellular protein release in bacterial suspensions. No effect was detected when bacteria were exposed to conventional heating or 200 W, whereas cell membrane integrity was slightly altered when cell suspensions were subjected to powers ranging from 400 to 2,000 W. Thermal characterization suggested that the temperature reached by the microwave-exposed samples for the contact time studied was not high enough to explain the measured modifications of cell membrane integrity. Because the results indicated that the cell response is power dependent, the hypothesis of a specific electromagnetic threshold effect, probably related to the temperature increase, can be advanced.
机译：这项研究的目的是研究2.45 GHz微波（MW）处理在各种条件下对大肠杆菌细胞膜的影响，将细胞悬浮液的平均温度保持在37°C，以检查可能的热对短期MW暴露的非热效应。为此，在仔细定义和控制的参数下对细菌进行微波照射，导致不连续的MW暴露，以将细菌细胞悬液的平均温度维持在37°C。将大肠杆菌细胞暴露于200至2,000 W的不连续微波（DW）处理不同的时间。对于每个实验，在37℃的水浴中进行常规加热（CH）作为对照。碘化丙啶（PI）染色后，除了评估细菌悬浮液中细胞内蛋白质的释放外，还使用流式细胞仪（FCM）研究了DW暴露对细胞膜的影响。当细菌暴露于常规加热或200 W的条件下未检测到任何作用，而当细胞悬液承受400至2,000 W的功率时，细胞膜的完整性略有改变。热表征表明，微波暴露的样品达到研究的接触时间不足以解释所测量的细胞膜完整性的修饰。由于结果表明电池响应与功率有关，因此可以提出可能与温度升高有关的特定电磁阈值效应的假设。

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