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首页> 外文期刊>Applied Microbiology >Dose-Dependent Effects of Lactobacillus rhamnosus on Serum Interleukin-17 Production and Intestinal T-Cell Responses in Pigs Challenged with Escherichia coli
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Dose-Dependent Effects of Lactobacillus rhamnosus on Serum Interleukin-17 Production and Intestinal T-Cell Responses in Pigs Challenged with Escherichia coli

机译：鼠李糖乳杆菌对大肠杆菌感染猪的血清白细胞介素17产生和肠道T细胞反应的剂量依赖性影响。

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The mechanism underlying the dose effect of probiotics on ameliorating diarrhea has not been fully elucidated. Here, low (1 × 10~(9) CFU/ml) or high (1 × 10~(11) CFU/ml) doses of Lactobacillus rhamnosus ATCC 7469 were administered orally to piglets for 1 week before F4 (K88)-positive enterotoxigenic Escherichia coli (F4~(+) ETEC) challenge. Administration of a low, but not a high, dose of L. rhamnosus decreased the percentage of CD3~(+) CD4~(+) CD8~(?) T cells in the peripheral blood. Notably, transiently increased serum concentrations of interleukin-17A (IL-17A) were observed after F4~(+) ETEC challenge in pigs pretreated with a high dose of L. rhamnosus . Administration of L. rhamnosus increased the percentage of the small intestinal lamina propria CD3~(+) CD4~(+) CD8~(?) cells and Peyer's patch CD3~(+) CD4~(?) CD8~(?) and CD3~(?) CD4~(?) CD8~(+) cells. The percentage of ileal intraepithelial CD3~(+) CD4~(?) CD8~(+) cells increased only in the high-dose piglets. Administration of L. rhamnosus downregulated expression of ileal IL-17A after F4~(+) ETEC challenge but had no effect on expression of gamma interferon (IFN-γ), IL-12, IL-4, and FOXP3 mRNA in the small intestine. Expression of jejunal IL-2, ileal transforming growth factor β1 (TGF-β1), and ileal IL-10 was upregulated in the low-dose piglets after F4~(+) ETEC challenge. Our findings suggest that amelioration of infectious diarrhea in piglets by L. rhamnosus is associated with the generation of lamina propria CD3~(+) CD4~(+) CD8~(?) T cells, the expansion of Peyer's patch CD3~(+) CD4~(?) CD8~(?) and CD3~(?) CD4~(?) CD8~(+) cells, and the attenuation of F4~(+) ETEC-induced increase in CD3~(+) CD4~(+) CD8~(+) T cells in the small intestine. However, consumption of high doses of L. rhamnosus may increase levels of serum IL-17A after F4~(+) ETEC challenge, thus eliciting a strong proinflammatory response.
机译：益生菌缓解腹泻的剂量作用的潜在机制尚未完全阐明。在这里，在F4（K88）阳性之前，对仔猪口服小剂量（1×10〜（9）CFU / ml）或高剂量（1×10〜（11）CFU / ml）的鼠李糖乳杆菌ATCC 7469。肠毒素大肠杆菌（F4〜（+）ETEC）攻击。给予低剂量但不是高剂量的鼠李糖乳杆菌可降低外周血中CD3〜（+）CD4〜（+）CD8〜（β）T细胞的百分比。值得注意的是，在用高剂量鼠李糖乳杆菌预处理的猪中，在F4〜（+）ETEC攻击后，观察到血清白介素17A（IL-17A）的瞬时升高。鼠李糖乳杆菌的施用增加了小肠固有层CD3〜（+）CD4〜（+）CD8〜（？）细胞和Peyer's patch CD3〜（+）CD4〜（？）CD8〜（？）和CD3的百分比〜（？）CD4〜（？）CD8〜（+）细胞。回肠上皮内CD3〜（+）CD4〜（？）CD8〜（+）细胞的百分比仅在大剂量仔猪中增加。鼠李糖乳杆菌的施用在F4〜（+）ETEC攻击后下调了回肠IL-17A的表达，但对小肠中γ-干扰素（IFN-γ），IL-12，IL-4和FOXP3 mRNA的表达没有影响。 F4〜（+）ETEC攻击后小剂量仔猪空肠IL-2，回肠转化生长因子β1（TGF-β1）和回肠IL-10的表达上调。我们的发现表明，鼠李糖乳杆菌改善仔猪的传染性腹泻与固有层CD3〜（+）CD4〜（+）CD8〜（？）T细胞的产生，派伊尔氏淋巴结膜CD3〜（+）的扩张有关。 CD4〜（？）CD8〜（？）和CD3〜（？）CD4〜（？）CD8〜（+）细胞，以及F4〜（+）ETEC诱导的CD3〜（+）CD4〜（+）的衰减+）小肠中的CD8〜（+）T细胞。然而，大剂量鼠李糖乳杆菌的摄入可能会增加F4〜（+）ETEC攻击后血清IL-17A的水平，从而引起强烈的促炎反应。

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