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首页> 外文期刊>Applied Microbiology >Activity and Viability of Methanogens in Anaerobic Digestion of Unsaturated and Saturated Long-Chain Fatty Acids
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Activity and Viability of Methanogens in Anaerobic Digestion of Unsaturated and Saturated Long-Chain Fatty Acids

机译：产甲烷菌在不饱和和饱和长链脂肪酸厌氧消化中的活性和活力

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Lipids can be anaerobically digested to methane, but methanogens are often considered to be highly sensitive to the long-chain fatty acids (LCFA) deriving from lipids hydrolysis. In this study, the effect of unsaturated (oleate [C_(18:1)]) and saturated (stearate [C_(18:0)] and palmitate [C_(16:0)]) LCFA toward methanogenic archaea was studied in batch enrichments and in pure cultures. Overall, oleate had a more stringent effect on methanogens than saturated LCFA, and the degree of tolerance to LCFA was different among distinct species of methanogens. Methanobacterium formicicum was able to grow in both oleate- and palmitate-degrading enrichments (OM and PM cultures, respectively), whereas Methanospirillum hungatei only survived in a PM culture. The two acetoclastic methanogens tested, Methanosarcina mazei and Methanosaeta concilii , could be detected in both enrichment cultures, with better survival in PM cultures than in OM cultures. Viability tests using live/dead staining further confirmed that exponential growth-phase cultures of M. hungatei are more sensitive to oleate than are M. formicicum cultures; exposure to 0.5 mM oleate damaged 99% ± 1% of the cell membranes of M. hungatei and 53% ± 10% of the cell membranes of M. formicicum . In terms of methanogenic activity, M. hungatei was inhibited for 50% by 0.3, 0.4, and 1 mM oleate, stearate, and palmitate, respectively. M. formicicum was more resilient, since 1 mM oleate and >4 mM stearate or palmitate was needed to cause 50% inhibition on methanogenic activity.
机译：脂质可以被厌氧消化成甲烷，但是产甲烷菌通常被认为对源自脂质水解的长链脂肪酸（LCFA）高度敏感。在这项研究中，分批研究了不饱和（油酸酯[C_（18：1）]）和饱和（硬脂酸酯[C_（18：0）]和棕榈酸酯[C_（16：0）]）LCFA对产甲烷古菌的影响。丰富和纯净的文化。总体而言，油酸盐对产甲烷菌的作用比饱和LCFA更为严格，不同产甲烷菌的物种对LCFA的耐受程度也不同。甲酸甲烷杆菌能够在降解油酸盐和棕榈酸酯的浓缩液中生长（分别为OM和PM培养），而饥饿的Methanospirillum Hangatei仅在PM培养物中存活。在两种富集培养物中均可以检测到两种测试的破乳产甲烷菌，即马氏甲烷八叠球菌（Methanosarcina mazei）和conc虫甲烷菌（Methanosaeta conciliii），在PM培养物中的存活率比在OM培养物中更高。使用活/死染色法进行的生存力测试进一步证实，与普通分枝杆菌培养相比，匈牙利分枝杆菌的指数生长期培养物对油酸盐更为敏感。暴露于0.5 mM的油酸盐中会损坏M. hangatei的细胞膜的99％±1％和formicicum的M.膜的53％±10％。在产甲烷活性方面，分别以0.3、0.4和1 mM的油酸酯，硬脂酸酯和棕榈酸酯将M. hungatei抑制50％。由于需要1 mM油酸酯和> 4 mM硬脂酸酯或棕榈酸酯才能对产甲烷活性产生50％的抑制作用，因此M. formicicum更具弹性。

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