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首页> 外文期刊>Applied Microbiology >Metabolic Engineering of a Glycerol-Oxidative Pathway in Lactobacillus panis PM1 for Utilization of Bioethanol Thin Stillage: Potential To Produce Platform Chemicals from Glycerol
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Metabolic Engineering of a Glycerol-Oxidative Pathway in Lactobacillus panis PM1 for Utilization of Bioethanol Thin Stillage: Potential To Produce Platform Chemicals from Glycerol

机译：乳酸杆菌PM1中甘油氧化途径的代谢工程，用于利用生物乙醇稀釜馏物：从甘油生产平台化学品的潜力

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Lactobacillus panis PM1 has the ability to produce 1,3-propanediol (1,3-PDO) from thin stillage (TS), which is the major waste material after bioethanol production, and is therefore of significance. However, the fact that L. panis PM1 cannot use glycerol as a sole carbon source presents a considerable problem in terms of utilization of this strain in a wide range of industrial applications. Accordingly, L. panis PM1 was genetically engineered to directly utilize TS as a fermentable substrate for the production of valuable platform chemicals without the need for exogenous nutrient supplementation (e.g., sugars and nitrogen sources). An artificial glycerol-oxidative pathway, comprised of glycerol facilitator, glycerol kinase, glycerol 3-phosphate dehydrogenase, triosephosphate isomerase, and NADPH-dependent aldehyde reductase genes of Escherichia coli , was introduced into L. panis PM1 in order to directly utilize glycerol for the production of energy for growth and value-added chemicals. A pH 6.5 culture converted glycerol to mainly lactic acid (85.43 mM), whereas a significant amount of 1,3-propanediol (59.96 mM) was formed at pH 7.5. Regardless of the pH, ethanol (82.16 to 83.22 mM) was produced from TS fermentations, confirming that the artificial pathway metabolized glycerol for energy production and converted it into lactic acid or 1,3-PDO and ethanol in a pH-dependent manner. This study demonstrates the cost-effective conversion of TS to value-added chemicals by the engineered PM1 strain cultured under industrial conditions. Thus, application of this strain or these research findings can contribute to reduced costs of bioethanol production.
机译：潘氏乳杆菌PM1具有从稀釜馏物（TS）生产1,3-丙二醇（1,3-PDO）的能力，稀釜馏物是生物乙醇生产后的主要废料，因此具有重要意义。然而，在广泛的工业应用中，就该菌株的利用而言，潘氏乳杆菌PM1不能使用甘油作为唯一碳源的事实提出了相当大的问题。因此，对潘氏乳酸杆菌PM1进行了基因工程改造，以直接利用TS作为可发酵的底物来生产有价值的平台化学品，而无需外源营养补充剂（例如糖和氮源）。为了将甘油直接用于甘油三酯PM1，将由甘油促进剂，甘油激酶，甘油3-磷酸脱氢酶，甘油三糖异构酶和NADPH依赖的醛还原酶基因组成的人工甘油氧化途径引入大肠杆菌。生产用于增长的能源和增值化学品。 pH 6.5培养物将甘油主要转化为乳酸（85.43 mM），而在pH 7.5形成大量的1,3-丙二醇（59.96 mM）。无论pH值如何，TS发酵都会产生乙醇（82.16至83.22 mM），这证实了人工途径代谢了甘油以产生能量，并以pH依赖的方式将其转化为乳酸或1,3-PDO和乙醇。这项研究表明，通过在工业条件下培养的工程PM1菌株，可以经济高效地将TS转化为增值化学品。因此，该菌株或这些研究结果的应用可以有助于降低生物乙醇生产的成本。

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