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首页> 外文期刊>Applied Microbiology >Expression and Physiological Role of Three Myxococcus xanthus Copper-Dependent P1B-Type ATPases during Bacterial Growth and Development
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Expression and Physiological Role of Three Myxococcus xanthus Copper-Dependent P1B-Type ATPases during Bacterial Growth and Development

机译：细菌生长发育过程中三种粘球菌铜依赖的P1B型ATPase的表达及其生理作用

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摘要

Myxococcus xanthus is a soil-dwelling bacterium that exhibits a complex life cycle comprising social behavior, morphogenesis, and differentiation. In order to successfully complete this life cycle, cells have to cope with changes in their environment, among which the presence of copper is remarkable. Copper is an essential transition metal for life, but an excess of copper provokes cellular damage by oxidative stress. This dual effect forces the cells to maintain a tight homeostasis. M. xanthus encodes a large number of genes with similarities to others reported previously to be involved in copper homeostasis, most of which are redundant. We have identified three genes that encode copper-translocating P_(1B)-ATPases (designated copA , copB , and copC ) that exhibit the sequence motifs and modular organizations of those that extrude Cu~(+). The expression of the ATPase copC has not been detected, but copA and copB are differentially regulated by the addition of external copper. However, while copB expression peaks at 2 h, copA is expressed at higher levels, and the maximum is reached much later. The fact that these expression profiles are nearly identical to those exhibited by the multicopper oxidases cuoA and cuoB suggests that the pairs CuoB-CopB and CuoA-CopA sequentially function to detoxify the cell. The deletion of any ATPase alters the expression profiles of other genes involved in copper homeostasis, such as the remaining ATPases or the Cus systems, yielding cells that are more resistant to the metal.
机译：Xanthus粘球菌是一种土壤细菌，具有复杂的生命周期，包括社会行为，形态发生和分化。为了成功完成此生命周期，电池必须应对其环境的变化，其中铜的存在非常明显。铜是生命中必不可少的过渡金属，但过量的铜会因氧化应激而引起细胞损伤。这种双重作用迫使细胞维持紧密的体内平衡。 Xanthus编码大量基因，这些基因与以前报道的参与铜稳态的其他基因具有相似性，其中大多数是多余的。我们已经确定了三个编码铜转运P_（1B）-ATPases（指定为copA，copB和copC）的基因，这些基因表现出挤出Cu〜（+）的序列基序和模块组织。尚未检测到ATPase copC的表达，但是copA和copB通过添加外部铜而受到差异调节。但是，虽然copB表达在2 h达到峰值，但copA却以更高的水平表达，并且最大值要晚得多。这些表达谱与多铜氧化酶cuoA和cuoB展示的表达谱几乎相同的事实表明，CuoB-CopB和CuoA-CopA对依次发挥了对细胞进行解毒的作用。任何ATP酶的缺失都会改变参与铜稳态的其他基因的表达谱，例如其余的ATP酶或Cus系统，从而产生对金属更具抵抗力的细胞。

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