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首页> 外文期刊>Applied Microbiology >From Waste to Plastic: Synthesis of Poly(3-Hydroxypropionate) in Shimwellia blattae
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From Waste to Plastic: Synthesis of Poly(3-Hydroxypropionate) in Shimwellia blattae

机译：从废物到塑料：Shimwellia blattae中聚（3-羟基丙酸酯）的合成

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摘要

In recent years, glycerol has become an attractive carbon source for microbial processes, as it accumulates massively as a by-product of biodiesel production, also resulting in a decline of its price. A potential use of glycerol in biotechnology is the synthesis of poly(3-hydroxypropionate) [poly(3HP)], a biopolymer with promising properties which is not synthesized by any known wild-type organism. In this study, the genes for 1,3-propanediol dehydrogenase ( dhaT ) and aldehyde dehydrogenase ( aldD ) of Pseudomonas putida KT2442, propionate-coenzyme A (propionate-CoA) transferase ( pct ) of Clostridium propionicum X2, and polyhydroxyalkanoate (PHA) synthase ( phaC1 ) of Ralstonia eutropha H16 were cloned and expressed in the 1,3-propanediol producer Shimwellia blattae . In a two-step cultivation process, recombinant S. blattae cells accumulated up to 9.8% ± 0.4% (wt/wt [cell dry weight]) poly(3HP) with glycerol as the sole carbon source. Furthermore, the engineered strain tolerated the application of crude glycerol derived from biodiesel production, yielding a cell density of 4.05 g cell dry weight/liter in a 2-liter fed-batch fermentation process.
机译：近年来，甘油已成为微生物过程中有吸引力的碳源，因为它作为生物柴油生产的副产品大量积累，因此价格也有所下降。甘油在生物技术中的潜在用途是合成聚（3-羟基丙酸酯）[poly（3HP）]，这是一种具有前景的生物聚合物，任何已知的野生型生物都无法合成。在这项研究中，恶臭假单胞菌KT2442的1,3-丙二醇脱氢酶（dhaT）和醛脱氢酶（aldD）的基因，丙酸梭菌X2的丙酸辅酶A（丙酸CoA）转移酶（pct）和聚羟基链烷酸（PHA）的基因。克隆了Ralstonia eutropha H16的合酶（phaC1），并在1,3-丙二醇生产商Shimwellia blattae中表达。在两步培养过程中，以甘油为唯一碳源的重组S. blattae细胞积累了高达9.8％±0.4％（wt / wt [细胞干重]）的poly（3HP）。此外，该工程菌株耐受了源自生物柴油生产的粗甘油的应用，在2升分批补料发酵过程中，细胞密度为4.05 g细胞干重/升。

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