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首页> 外文期刊>Applied Microbiology >Imidazoacridinone Derivatives as Efficient Sensitizers in Photoantimicrobial Chemotherapy
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Imidazoacridinone Derivatives as Efficient Sensitizers in Photoantimicrobial Chemotherapy

机译：咪唑并rid啶酮衍生物作为光抗菌化学疗法中的有效增敏剂

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The objective of this study was to investigate a new potential photosensitizer (PS) in the photodynamic inactivation (PDI) of microorganisms in vitro (11 reference strains and 13 clinical isolates, representing common Gram-positive and Gram-negative human pathogens), with special emphasis on Candida albicans . We studied the light-induced cytotoxicity of the imidazoacridinone derivative C1330 toward fungal cells grown in planktonic form. We examined the influence of various parameters (time of incubation, PDI quencher effect, and C1330 accumulation in C. albicans cells) on the efficacy of light-dependent cytotoxicity. Additionally, we checked for the potential cyto- and phototoxic activity of C1330 against human dermal keratinocytes. In our research, we used a broadband incoherent blue light source (380 to 470 nm) with an output power of 100 mW/cm~(2). In vitro studies showed that the C1330 action against C. albicans was a light-dependent process. C1330 was an efficient photosensitizer in the photodynamic inactivation of C. albicans , which reduced the growth of planktonic cells by 6.1 log_(10) units. Efficient accumulation of PS in the nucleus and vacuoles was observed after 30 min of incubation, which correlated with the highest photokilling efficacy. Significant changes in intracellular structure were observed upon illumination of C1330-incubated C. albicans cells. In the case of the human HaCaT cell line, approximately 40% of cells survived the treatment, which indicates the potential benefit of further study of the application of C1330 in photoantimicrobial chemotherapy. These data suggest that PDI may be a viable approach for the treatment of localized C. albicans infections.
机译：这项研究的目的是研究一种新的潜在的光敏剂（PS），用于体外微生物的光动力学灭活（PDI）（11种参考菌株和13种临床分离株，代表常见的革兰氏阳性和革兰氏阴性人类病原体），并具有特殊意义。强调白色念珠菌。我们研究了咪唑并rid啶酮衍生物C1330对浮游形式生长的真菌细胞的光诱导细胞毒性。我们检查了各种参数（孵育时间，PDI淬灭作用和白色念珠菌细胞中C1330积累）对光依赖性细胞毒性功效的影响。此外，我们检查了C1330对人皮肤角质形成细胞的潜在细胞毒性和光毒性活性。在我们的研究中，我们使用了宽带非相干蓝光光源（380至470 nm），其输出功率为100 mW / cm〜（2）。体外研究表明，C1330对白色念珠菌的作用是一个光依赖性过程。 C1330是白色念珠菌的光动力学灭活的有效光敏剂，可将浮游细胞的生长减少6.1 log_（10）单位。孵育30分钟后，观察到PS在细胞核和液泡中的有效积累，这与最高的光杀死效力相关。照明C1330培养的白色念珠菌细胞后，观察到细胞内结构发生了显着变化。对于人类HaCaT细胞系，大约40％的细胞在治疗后存活了下来，这表明了进一步研究C1330在光抗微生物化学疗法中的应用的潜在益处。这些数据表明，PDI可能是治疗局部白色念珠菌感染的可行方法。

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