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首页> 外文期刊>Applied Microbiology >Anaerobic α-Amylase Production and Secretion with Fumarate as the Final Electron Acceptor in Saccharomyces cerevisiae
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Anaerobic α-Amylase Production and Secretion with Fumarate as the Final Electron Acceptor in Saccharomyces cerevisiae

机译：酿酒酵母中厌氧α-淀粉酶的产生和富马酸酯的分泌作为最终电子受体

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In this study, we focus on production of heterologous α-amylase in the yeast Saccharomyces cerevisiae under anaerobic conditions. We compare the metabolic fluxes and transcriptional regulation under aerobic and anaerobic conditions, with the objective of identifying the final electron acceptor for protein folding under anaerobic conditions. We find that yeast produces more amylase under anaerobic conditions than under aerobic conditions, and we propose a model for electron transfer under anaerobic conditions. According to our model, during protein folding the electrons from the endoplasmic reticulum are transferred to fumarate as the final electron acceptor. This model is supported by findings that the addition of fumarate under anaerobic (but not aerobic) conditions improves cell growth, specifically in the α-amylase-producing strain, in which it is not used as a carbon source. Our results provide a model for the molecular mechanism of anaerobic protein secretion using fumarate as the final electron acceptor, which may allow for further engineering of yeast for improved protein secretion under anaerobic growth conditions.
机译：在这项研究中，我们专注于在厌氧条件下在酿酒酵母中生产异源α-淀粉酶。我们比较了有氧和厌氧条件下的代谢通量和转录调控，目的是确定在厌氧条件下蛋白质折叠的最终电子受体。我们发现酵母在厌氧条件下比在有氧条件下产生的淀粉酶更多，并且我们提出了在厌氧条件下电子转移的模型。根据我们的模型，在蛋白质折叠过程中，来自内质网的电子被转移到富马酸酯作为最终的电子受体。该模型得到以下发现的支持：在厌氧（但不是好氧）条件下添加富马酸酯可改善细胞生长，特别是在不用作碳源的α-淀粉酶生产菌株中。我们的结果为使用富马酸酯作为最终电子受体的厌氧蛋白质分泌的分子机制提供了一个模型，该模型可允许进一步改造酵母以在厌氧生长条件下改善蛋白质的分泌。

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