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首页> 外文期刊>Applied Microbiology >Diversity and Antimicrobial Resistance of Salmonella enterica Isolates from Surface Water in Southeastern United States
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Diversity and Antimicrobial Resistance of Salmonella enterica Isolates from Surface Water in Southeastern United States

机译：美国东南部地表水肠炎沙门氏菌分离物的多样性和抗药性

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A study of prevalence, diversity, and antimicrobial resistance of Salmonella enterica in surface water in the southeastern United States was conducted. A new scheme was developed for recovery of Salmonella from irrigation pond water and compared with the FDA's Bacteriological Analytical Manual (8th ed., 2014) (BAM) method. Fifty-one isolates were recovered from 10 irrigation ponds in produce farms over a 2-year period; nine Salmonella serovars were identified by pulsed-field gel electrophoresis analysis, and the major serovar was Salmonella enterica serovar Newport ( S . Newport, n = 29), followed by S. enterica serovar Enteritidis ( n = 6), S. enterica serovar Muenchen ( n = 4), S. enterica serovar Javiana ( n = 3), S. enterica serovar Thompson ( n = 2), and other serovars. It is noteworthy that the PulseNet patterns of some of the isolates were identical to those of the strains that were associated with the S . Thompson outbreaks in 2010, 2012, and 2013, S . Enteritidis outbreaks in 2011 and 2013, and an S . Javiana outbreak in 2012. Antimicrobial susceptibility testing confirmed 16 S . Newport isolates of the multidrug resistant-AmpC (MDR-AmpC) phenotype, which exhibited resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (ACSSuT), and to the 1st, 2nd, and 3rd generations of cephalosporins (cephalothin, amoxicillin-clavulanic acid, and ceftriaxone). Moreover, the S . Newport MDR-AmpC isolates had a PFGE pattern indistinguishable from the patterns of the isolates from clinical settings. These findings suggest that the irrigation water may be a potential source of contamination of Salmonella in fresh produce. The new Salmonella isolation scheme significantly increased recovery efficiency from 21.2 (36/170) to 29.4% (50/170) ( P = 0.0002) and streamlined the turnaround time from 5 to 9 days with the BAM method to 4 days and thus may facilitate microbiological analysis of environmental water.
机译：对美国东南部地表水中肠炎沙门氏菌的患病率，多样性和抗药性进行了研究。开发了一种新方案，用于从灌溉池塘水中回收沙门氏菌，并与FDA的《细菌学分析手册》（2014年第8版）（BAM）方法进行了比较。在2年的时间里，从农产品农场的10个灌溉池塘中回收了51株分离株。通过脉冲场凝胶电泳分析鉴定出九种沙门氏菌血清型，主要血清型为肠沙门氏菌纽波特沙门氏菌（纽波特，n = 29），其次为肠炎沙门氏菌肠炎沙门氏菌（n = 6），肠沙门氏菌血清型慕尼黑。 （n = 4），肠炎沙门氏菌血清型Javiana（n = 3），肠炎沙门氏菌血清型汤普森（n = 2）和其他血清型。值得注意的是，某些分离株的PulseNet模式与与S.相关的菌株相同。汤普森（Thompson）在2010、2012和2013年爆发S。 2011年和2013年爆发肠炎沙门氏菌，S爆发。 2012年爆发哈维亚病毒。抗药性测试确认为16S。纽波特分离株具有多重耐药性AmpC（MDR-AmpC）表型，对氨苄青霉素，氯霉素，链霉素，磺胺甲恶唑和四环素（ACSSuT）以及第一，第二和第三代头孢菌素（头孢菌素，阿莫西林-棒酸和头孢曲松）。此外，S。纽波特MDR-AmpC分离株的PFGE模式与临床环境中分离株的模式没有区别。这些发现表明，灌溉用水可能是新鲜农产品中沙门氏菌污染的潜在来源。新的沙门氏菌分离方案将回收率从21.2（36/170）显着提高到29.4％（50/170）（P = 0.0002），并将BAM方法的周转时间从5天缩短为9天，从而缩短了4天，因此可能有助于环境水的微生物学分析。

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