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Strain Design of Ashbya gossypii for Single-Cell Oil Production

机译：用于单细胞采油的Ashbya gossypii菌株设计

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Single-cell oil (SCO) represents a sustainable alternative for the oil industry. Accordingly, the identification of microorganisms with either higher lipidogenic ability or novel capacities for the transformation of raw materials constitutes a major challenge for the field of oil biotechnology. With this in mind, here, we were prompted to address the lipidogenic profile of the filamentous hemiascomycete Ashbya gossypii , which is currently used for the microbial production of vitamins. We found that A. gossypii mostly accumulates unsaturated fatty acids (FAs), with more than 50% of the total FA content corresponding to oleic acid. In addition, we engineered A. gossypii strains both lacking the beta-oxidation pathway and also providing ATP-citrate lyase (ACL) activity to block the degradation of FA and to increase the cytosolic acetyl-coenzyme A (CoA) content, respectively. The lipidogenic profile of the newly developed strains demonstrates that the mere elimination of the beta-oxidation pathway in A. gossypii triggers a significant increase in lipid accumulation that can reach 70% of cell dry weight. The use of A. gossypii as a novel and robust tool for the production of added-value oils is further discussed.
机译：单细胞油（SCO）代表了石油工业的可持续替代品。因此，鉴定具有更高脂质生成能力或具有新颖的原料转化能力的微生物构成了石油生物技术领域的主要挑战。考虑到这一点，在这里，我们被提示处理丝状半胱氨酸的阿什比亚棉球（Ashbya gossypii）的生脂特性，该材料目前用于微生物生产维生素。我们发现棉铃虫主要积累不饱和脂肪酸（FAs），其中总FA含量的50％以上与油酸相对应。此外，我们工程改造了棉铃虫菌株，它们既缺乏β-氧化途径，又提供ATP柠檬酸裂合酶（ACL）活性，分别阻断FA的降解并增加胞浆乙酰辅酶A（CoA）的含量。新开发菌株的生脂特性表明，棉孢曲霉中β-氧化途径的消除消除了脂质积累的显着增加，可以达到细胞干重的70％。进一步讨论了使用棉球菌作为生产增值油的新颖而强大的工具。

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